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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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相关实验视频

Updated: Jul 2, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

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开源显微镜附加组件用于结构化照明显微镜.

Mélanie T M Hannebelle1,2,3, Esther Raeth1,2, Samuel M Leitao1

  • 1School of Engineering, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.

Nature communications
|February 20, 2024
PubMed
概括
此摘要是机器生成的。

研究人员现在可以通过openSIM附加程序访问超分辨率显微镜. 这种开放硬件系统升级了现有的显微镜,使先进的成像可用于创新的研究,而不需要新的设备.

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
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科学领域:

  • 显微镜的使用方法
  • 光学成像技术的成像
  • 生物技术是生物技术.

背景情况:

  • 超分辨率显微镜提供了先进的成像能力,但通常需要专门的,昂贵的系统.
  • 许多研究实验室缺乏获得或制造专用超高分辨率显微镜的资源.
  • 这限制了科学界很大一部分人使用尖端技术的机会.

研究的目的:

  • 为超分辨率显微镜引入一种可访问的解决方案.
  • 让没有专用系统的研究人员能够进行先进的成像.
  • 通过民主化超分辨率技术,促进创新研究.

主要方法:

  • 开发一个开放硬件附加模块openSIM的开发.
  • 设计和文档,以便轻松重复开放SIM系统.
  • 将openSIM与现有的光学显微镜集成,以创建结构化照明显微镜 (SIM).

主要成果:

  • 开放SIM成功地升级了标准光学显微镜,以执行超分辨率成像.
  • 开放硬件设计允许在其他实验室直接复制.
  • 这种方法使得超分辨率显微镜更容易获得和更具成本效益.

结论:

  • 开放SIM附加程序显著提高现有实验室设备的功能.
  • 这一创新使超高分辨率显微镜实现了民主化,赋予了更多研究人员权力.
  • 通过降低超分辨率技术的进入壁垒,促进了先进的科学发现.