阴道微生物组在各种生育治疗期间发生变化
M M van den Tweel1,2, E H A van den Munckhof3, M van der Zanden2
1Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, The Netherlands.
Reproductive sciences (Thousand Oaks, Calif.)
|February 21, 2024
概括
诸如体外受精 (IVF) 等生育程序期间的激素治疗可能会对阴道微生物组产生负面影响,减少有益的乳酸菌. 这可能会影响怀孕的结果,这表明调整治疗时间或策略可能有好处.
科学领域:
- 生殖内分泌学和不孕不育问题
- 微生物组研究 微生物组研究
- 妇科健康 妇科健康
背景情况:
- 细菌性阴道炎 (BV) 与降低生育能力和不良妊娠结果有关,特别是在接受辅助生殖技术的妇女中.
- 在生育治疗中使用的激素刺激方案对阴道微生物组合的影响仍然不完全理解.
研究的目的:
- 研究激素治疗在生育治疗期间对阴道微生物群的影响,重点关注乳酸菌丰度的变化.
- 探索阴道微生物组变化与经过宫内授精 (IUI) 和体外受精 (IVF) 的个体怀孕结果之间的关系.
主要方法:
- 一项前性队列研究,涉及2019年7月至2022年8月期间接受生育治疗 (IUI,IVF,ICSI) 的53对夫妇.
- 在治疗周期期间在多个时间点收集的阴道样本.
- 定量PCR (qPCR) 用于BV检测和16S rRNA基因测序用于微生物组分析.
主要成果:
- 在生育治疗期间观察到乳酸菌百分比显著下降,这表明阴道微生物组发生了变化.
- 24%的参与者通过qPCR检测出BV阳性,其中9人在治疗期间从BV阴性转变为BV阳性.
- 两组之间或随着时间的推移,尚农多样性指数没有发现显著差异,尽管在那些患有BV的人群中发现了更高多样性的趋势.
结论:
- 在生育设置中的荷尔蒙治疗可以导致阴道失生症,其特点是乳酸菌减少,可能会影响治疗成功.
- 在生育治疗期间监测阴道微生物群状态可能是有益的.
- 考虑治疗时间,生活方式调整或"结一切"策略可以减轻阴道微生物组恶化的负面影响.
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