细胞染色体P450 CitCYP97B通过氧化酸在类植物中的β-cryptoxanthin来调节胡卜素积累多样性
Yingzi Zhang1, Jiajing Jin1, Nan Wang1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Plant communications
|February 21, 2024
概括
研究人员确定了基因CitCYP97B,该基因控制类水果中的β-素含量. 这种基因的遗传变异影响了β-密桑的积累,为生物强化生产提供了目标.
科学领域:
- 植物生物化学和遗传学
- 营养科学和食品生物强化
背景情况:
- 胡卜素,像β-cryptoxanthin一样,是维生素A的重要化合物,存在于类水果中.
- 调节类植物中β-加密素积累的遗传机制在很大程度上仍未被描述.
研究的目的:
- 描述胡卜素积累和类植物中β-素的分类概况.
- 通过全基因组关联研究,揭示β-加密丁积累的遗传基础.
主要方法:
- 对65种胡卜素和衍生物进行广泛向的代谢分析.
- 全基因组关联研究 (GWAS) 使用81个新测序的和69个以前测序的类加入.
- 异质表达系统和植物实验验验证基因功能.
主要成果:
- 确定了CitCYP97B,一种新型的细胞染色体P450单氧化酶,作为调节β-加密丁的关键基因.
- 已证明CitCYP97B通过氧化它的β环来负面调节β-加密丁含量.
- 在CitCYP97B中发现了两个cis元素变异,与改变的β-密素水平有关,在进化过程中受到杂交/内进化的影响.
结论:
- 阐明了类植物中β-加密素积累的遗传调节,确定了CitCYP97B作为一个关键的负调节器.
- 提供了对塑造类植物中胡卜素多样性的进化机制的见解.
- 确立了CitCYP97B作为开发β-cryptoxanthin-biofortified类品种的潜在遗传标.
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