人类骨肌肉转录组和蛋白质组的与年龄相关的变化比初级衰老更容易受到慢性炎症和身体不活动的影响
Nadia S Kurochkina1, Mira A Orlova1, Maksim A Vigovskiy2
1Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
Aging cell
|February 21, 2024
概括
主要衰老影响的基因比二次衰老少,由不活动和炎症驱动. 这项研究区分了它们在骨肌肉分子变化中的作用,揭示了不同的基因调节模式.
科学领域:
- 分子生物学分子生物学
- 老年学是指老年学的学科.
- 骨肌肉生理学 骨肌肉生理学
背景情况:
- 区分初级衰老和二次衰老 (由炎症和不活动驱动) 对于理解与年龄相关的分子变化至关重要.
- 骨肌是受衰老,炎症和身体活动水平影响的关键组织.
研究的目的:
- 研究初级衰老和二级衰老因素 (慢性炎症,身体不活动) 在调节人类骨肌肉中的转录基因和蛋白质基因配置中的不同作用.
- 在骨关节炎的背景下,确定由初级衰老和二级衰老影响的特定基因和分子通路.
主要方法:
- 在骨肌样本中对基因表达 (转录组学) 和蛋白质概况 (蛋白质组学) 的比较分析.
- 包括年轻的健康人群,年轻的骨关节炎患者和年长的骨关节炎患者,以建模衰老和不活动/炎症的影响.
- 利用骨关节炎作为长期身体不活动和慢性炎症的模型.
主要成果:
- 在老年人中,与年龄相关的广泛基因表达变化 (约4000个基因) 主要与身体不活动和慢性炎症有关,而不是初级衰老.
- 主要衰老与参与DNA修复,RNA处理,转录,线粒体功能和蛋白质稳定的一组较小的基因 (约200个) 的调节有关.
- 与衰老相关的蛋白质调节主要发生在转录后水平.
结论:
- 身体不活动和慢性炎症显著推动骨肌肉中与年龄相关的分子变化,在许多方面掩盖了初级衰老的影响.
- 主要衰老影响与核和线粒体基因调节和蛋白质稳定相关的特定途径.
- 鉴定出基因和调节器为未来研究细胞衰老和衰老机制提供了资源.
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