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相关概念视频

CRISPR01:59

CRISPR

51.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K

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相关实验视频

Updated: Jul 2, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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基于CRISPR-Cas9的基因组插入技术的最新进展.

Xinwen Chen1,2, Jingjing Du1,2, Shaowei Yun1,2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

Molecular therapy. Nucleic acids
|February 21, 2024
PubMed
概括

本综述探讨了可编程基因组插入技术,包括基于CRISPR的方法. 它详细介绍了每个工具的优缺点,以指导研究人员进行基础研究和治疗应用.

关键词:
与CRISPR相关的转位子这是一种依赖DSB的内置.MT:编辑RNA/DNA的方法细胞和基因疗法细胞和基因疗法.主编辑主要编辑.可编程的基因组插入.

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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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相关实验视频

Last Updated: Jul 2, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 可编程的基因组插入对于生物研究和治疗开发至关重要.
  • 基于CRISPR的技术已经显著提升了基因组编辑能力.
  • 现有的方法通常依赖于细胞DNA修复通路,如HDR,NHEJ和MMEJ.

研究的目的:

  • 审查可编程基因组插入技术的最新进展.
  • 分析各种基因组插入工具的优缺点.
  • 确定改善基因组编辑技术的未来方向.

主要方法:

  • 关于可编程基因组插入策略的最新文献摘要.
  • 对诸如原始编辑,整合酶介导插入和CRISPR相关转位子等技术的比较分析.
  • 讨论不同方法的效率,目标范围和特异性.

主要成果:

  • 基于CRISPR的基因组插入策略正在迅速发展.
  • 像原始编辑和CRISPR相关的转子体这样的新工具扩大了精确基因组修改的可能性.
  • 每种技术都有独特的优势和局限性,影响其应用.

结论:

  • 对基因组插入工具的知情选择对于成功的研究和治疗应用至关重要.
  • 正在进行的努力重点是提高编辑效率,特异性和准范围.
  • 未来的改进有望促进基本的生物学理解和临床治疗.