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在PgABCC2的突变赋予Cry1Ac在粉红色虫的低水平的耐药性

Ling Wang¹, Min Xu², Lu He²

¹Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, China.

Pest management science

|February 21, 2024

概括

在ABC传送器PgABCC2中发生的突变使粉红色虫对Bacillus thuringiensis (Bt) Cry1Ac毒素产生抵抗力. 这一发现对于管理转基因作物的害虫耐药性至关重要.

科学领域:

分子生物学分子生物学
昆虫学昆虫学是一门学科.
生物化学生化学

背景情况:

增加害虫对Bacillus thuringiensis (Bt) 作物的耐药性需要了解耐药性机制.
以前的研究将粉红色虫对Cry1Ac的耐药性与cadherin基因PgCad1.1.中的突变联系起来.
这项研究研究了ABC传递器PgABCC2作为潜在的Cry1Ac受体的作用.

研究的目的:

为了测试ABC传送器PgABCC2作为Bt Cry1Ac毒素的受体的假设.
为了识别和表征PgABCC2中的突变,与粉红色虫中的Cry1Ac耐药性相关.

主要方法:

在实验室选择的耐药菌株中识别和表征一种新的PgABCC2耐药性等位基因 (rC2).
基因分析以确定Cry1Ac抗性与PgABCC2.2的遗传模式和联系.
在培养的昆虫细胞中表达野生型和突变的PgABCC2,以评估蛋白质局部化和毒素结合.

主要成果:

确定了第一个PgABCC2耐药性等位基因 (rC2),具有框架转移突变,导致缺少NBD2域的截断蛋白质.
耐药菌株 (AQ-C2) 显示Cry1Ac耐药性增加了20.2倍,遗传性是递归的,与PgABCC2.2相关.
突变的PgABCC2在培养细胞中表现出降低的血局部化和降低的Cry1Ac毒性 (减少13.9倍).

结论:

证实PgABCC2是Bt Cry1Ac毒素的功能性受体.
失去PgABCC2的炭基末端,包括NBD2域,使粉红幼虫和培养细胞具有低水平的Cry1Ac耐药性.

关键词:

ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2A 细菌 thuringiensis 这种疾病.在Cry1AcAc.粉红色的虫粉红色的虫电阻机制的电阻机制.

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