在严重的COVID-19疾病中增加循环的血小板衍生的细胞外囊泡
Tuukka Helin1, Mari Palviainen2,3, Marja Lemponen1
1HUS Diagnostics Centre, HUSLAB Clinical Chemistry, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
Platelets
|February 21, 2024
概括
在COVID-19患者中,细胞外囊泡 (EVs) 和残留细胞 (RCs) 与凝血障碍相关. 在EV和RC上的血小板激活标记,特别是小型EV (sEV),显示了COVID-19进展的预后潜力.
科学领域:
- 血液学 血液学 血液学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 凝血障碍是COVID-19严重程度的关键.
- 细胞外囊泡 (EVs) 和残留细胞 (RCs) 在COVID-19相关的凝血病中的作用仍未得到充分研究.
- D-二聚合物水平是COVID-19中凝血激活的已确立标志物.
研究的目的:
- 调查中型电动汽车 (mEV),大型电动汽车/RC和小型电动汽车 (sEV) 在COVID-19病原性中的参与.
- 为了将EV和RC特征与D-二次体水平和血小板激活相关联.
- 探索COVID-19中特定的EV亚群的预后价值.
主要方法:
- 使用高灵敏度流细胞计量来量化mEVs和大型EVs/RCs在COVID-19患者 (按D-二次数水平分层) 和健康对照中.
- 分析了EVs和RCs的血小板 (CD61),红细胞 (CD235a),白细胞 (CD45),内皮细胞 (CD31) 和氨酸素暴露 (通过乳腺素结合) 的标志物.
- 单颗粒干扰度反射成像传感器技术用于sEV检测和分析CD41a (血小板标记物) 与EV标记物 (CD9,CD63,CD81) 的同位化.
主要成果:
- 与低D-二次体或对照患者相比,高D-二次体水平的患者表现出显著更高的RC和sEV数量.
- 增加的CD61+和乳腺林+mEVs和RCs水平与血小板激活和凝血障碍有关.
- 在CD41a+sEV上特定的四氨酸签名区分了低D-二次数的COVID-19患者与健康对照.
结论:
- 剩余细胞和细胞外囊泡,特别是SEVs,在COVID-19患者中升高,D-二次数水平高.
- 在EV和RC上的血小板激活标记与COVID-19相关的凝血异常有关.
- 循环的血小板衍生的sEV亚群可以作为COVID-19进展中的有价值的预后生物标志物.
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