增强B. licheniformis BF-002通过碳和源的自动共养来增强其分泌
Jian Ding1, Qingyuan Liu2, Wenbiao Hou1
1The key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, China.
Biotechnology and bioengineering
|February 21, 2024
概括
自动联合养策略优化了Bacillus licheniformis的培养,以提高子的产生. 这种方法可以提高子产量和细菌粉末的生存能力,从而实现有效的环境和农业应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 巴西菌的配方对于环境修复,肠道健康和土壤改善至关重要.
- 足够的子度对于B. licheniformis配方的疗效至关重要.
- 优化子生产是提高配方质量的关键.
研究的目的:
- 研究碳和源对B. licheniformis BF-002种植的协同作用.
- 开发一种自动联合养策略,以最大限度地提高子的产量.
- 评估该策略对子产量,生存能力和基因表达的影响.
主要方法:
- 培养B. licheniformis BF-002与控制的葡萄糖和氨基水平.
- 在5L和50L生物反应堆中实施自动联合养策略.
- 细菌的喷雾干燥和在各种条件下细胞活力的评估.
- 对关键子形成基因的转录水平的分析.
主要成果:
- 自动联合养策略显著增加了子产量,达到2.86 × 10^10 CFU/mL.
- 与固定的葡萄糖度相比,5L和50L尺度的子产量分别提高了6.81%和30.00%.
- 喷雾干燥的细菌粉末即使在恶劣的储存条件下也保持了高细胞活力 (85.94%和82.68%).
- 子形成基因 (spo0A, spoIIGA, bofA, spoIV) 的转录增加了7.42%9.79%.
结论:
- 开发的自动联合养策略有效地增强了Bacillus licheniformis的生长和子形成.
- 这一策略提高了B. licheniformis配方的质量和稳定性,用于各种应用.
- 通过联合养优化子生产对于最大限度地提高B. licheniformis在环境和农业环境中的益处至关重要.
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