OsCSN2通过调节GA和BR通路来调节Oryza sativa L.的生长和发育
Shining Han1, Weijie Yue1, Anar Bao1
1College of Life Sciences, Jilin Agricultural University, Changchun, 130118, People's Republic of China.
Functional & integrative genomics
|February 21, 2024
概括
米中的COP9信号酶子单元2 (CSN2) 通过影响DELLA蛋白SLR1降解来影响植物发育. OsCSN2中的突变影响耕种和开花时间,揭示了它在生长调节中的作用.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- COP9信号酶 (CSN) 对于植物生长和发育至关重要.
- CSN2是一种高度保护的子单元,但其在大米 (Oryza sativa L.) 中的特定作用尚不清楚.
研究的目的:
- 研究CSN2在米生长和发育中的作用.
- 分析OsCSN2突变和过度表达对大米表型的影响.
主要方法:
- 使用CRISPR/Cas9技术进行基因编辑,以创建oscsn2突变.
- 过度表达OsCSN2 (OsCSN2-OE) 和产生OsCSN2点突变 (OsCSN2K64E,OsCSN2K67E,OsCSN2K71E,OsCSN2K104E) 的过程.
- 现型观测和基因表达分析从幼苗到标题阶段.
主要成果:
- OsCSN2的修改在早期阶段表现出有限的影响,但在60天后有显著差异.
- 与OsCSN2-OE相比,OsCSN2点突变体表现出增加的耕作和早期的头部/开花.
- 突变影响了无处不在的部位,影响了DELLA蛋白SLR1降解和GA/BR通路.
结论:
- OsCSN2在调节耕和发展时间方面发挥着至关重要的作用.
- OsCSN2通过调节CSN复合体内的SLR1稳定性来影响植物生长.
- OsCSN2是大米中吉伯雷林 (GA) 和类固醇 (BR) 信号通路的关键调节者.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Meristems and Plant Growth
45.9K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
45.9K
Regulation of Transpiration by Stomata
28.3K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.3K
Short-distance Transport of Resources
16.0K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
16.0K
Key Elements for Plant Nutrition
18.7K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.7K
Regulation of Expression at Multiple Steps
907
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
907


