在基于生物信息学分析的hiPSCs-CMs中鉴定糖尿病心肌病的关键基因
Shuo An1,2,3, Hongchen Bi1, Xiaoli Luo1
1School of Medical Laboratory, Tianjin Medical University, Tianjin, 300203, China.
Molecular and cellular biochemistry
|February 21, 2024
概括
糖尿病心肌病 (DbCM) 是一种严重的糖尿病并发症. 这项研究确定了包括PGK1和ENO1在内的关键基因,为DbCM提供了潜在的新治疗点.
科学领域:
- 心血管研究研究心血管研究
- 基因组学就是基因组学.
- 代谢疾病 代谢疾病
背景情况:
- 糖尿病心肌病 (DbCM) 是糖尿病的主要血管并发症,导致心力衰竭.
- 对于DbCM及其关键基因的复杂病原体的理解尚不完全.
研究的目的:
- 使用生物信息学分析识别与糖尿病心肌病相关的新型差异表达基因 (DEGs) 和枢纽基因.
- 在DbCM的体外模型中验证已识别的枢纽基因的表达.
主要方法:
- 使用NCBI的GEO数据集 (GSE62203,GSE197850) 用于通过GEO2R进行DEG分析.
- 使用DAVID进行了基因本体学 (GO) 和KEGG通路丰富分析.
- 使用STRING和Cytoscape构建了蛋白质-蛋白质相互作用网络,以识别枢纽基因.
- 在用qRT-PCR刺激的AC16细胞中验证了枢纽基因表达.
主要成果:
- 在两个数据集之间确定了73个共同的DEG (47个调高,26个调低).
- 丰富分析突出了与新陈代谢,缺氧,亡和细胞增殖相关的途径.
- 已经确定了前十个核心基因:LDHA,PGK1,SLC2A1,ENO1,PFKFB3,EGLN1,MYC,PDK1,EGLN3,BNIP3. 这些基因是最重要的.
- 实验室验证显示PGK1,SLC2A1,PFKFB3,EGLN1,MYC,EGLN3,BNIP3和ENO1的表达变化,LDHA没有变化.
结论:
- 这项研究确定了关键的DEG和枢纽基因,这些基因与DbCM病原发生有关.
- 据报道,PGK1和ENO1是针对性DbCM治疗的潜在候选基因.
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