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Updated: Jul 2, 2025

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

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在细胞和组织中通过BaseScopeTM战略进行circRNAs的现场杂交

Eleonora D'Ambra1, Erika Vitiello2,3, Tiziana Santini3

  • 1Center for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), Rome, Italy.

Methods in molecular biology (Clifton, N.J.)
|February 21, 2024
PubMed
概括

这项研究引入了一种先进的RNA-FISH方法来检测循环RNA (circRNA),这对于了解细胞发育和疾病至关重要. 该BaseScopeTM检测提供了一种高度敏感和特定的方式来可视化细胞和组织中的这些具有挑战性的分子.

关键词:
这是BaseScopeTM的基础.循环RNA 是一个循环RNA.同焦点显微镜的共聚焦显微镜免疫光效应 免疫光效应在现场混合化.这些神经元是神经元.在RNA-FISH中使用.超高分辨率显微镜的使用方法在ncRNA中,我们可以

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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