相关实验视频
Updated: Jul 2, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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循环RNA的纳米孔介导测序
Morten Trillingsgaard Venø1, Junyi Su2, Yan Yan1
1Omiics ApS, Aarhus, Denmark.
Methods in molecular biology (Clifton, N.J.)
|February 21, 2024
概括
本研究引入了长读测序 (LRS) 方法,以充分描述圆形RNA (circRNA) 和它们独特的外子-内子结构. 这些技术使得可以在背接接口进行精确的测序,以便进行全面的circRNA分析.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 在RNA生物学,RNA生物学.
背景情况:
- 循环RNAs (circRNAs) 是由独特的背接事件形成的RNA分子,在5'和3'端之间形成共价键.
- 大多数circRNA都由线性拼接的多个外子组成,但它们的完整外子-内子结构,特别是跨越背拼接接口,仍然难以全面测序.
- 准确地描述circRNA结构对于理解它们的生物发生和功能至关重要.
研究的目的:
- 描述用于测序全长circRNA的强大方法,包括背接接口.
- 通过使用长读序列 (LRS) 实现特定于circRNA的外子-内子结构的明确表征.
- 为全面的circRNA分析提供两个不同的LRS策略.
主要方法:
- 该协议详细介绍了两种长读测序 (LRS) 方法:circNick-LRS用于全球circRNA测序和circPanel-LRS用于针对性测序选定的circRNAs.
- circNick-LRS涉及从总RNA中丰富circRNA,然后进行测序.
- circPanel-LRS允许对预先选择的circRNA面板进行测序,而无需事先进行丰富.
主要成果:
- 这些LRS方法允许在背接接口上对全长circRNA进行测序.
- 实现了对circRNA特异性外子-内子结构的明确表征.
- 这些方法已经成功地用于表征小鼠和人类大脑中的超过10,000个circRNAs,如卡里姆等人所描述的那样. (拉希米等人,2021年中天共12:4825).
结论:
- 使用LRS描述的circRNA测序协议为详细的结构分析提供了强大的工具.
- 这些方法有助于对circRNA外子-内子结构的明确表征.
- 这些技术的应用显著推进了研究circRNA生物学和功能的研究.
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