相关实验视频

Updated: Jul 2, 2025

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Published on: November 30, 2018

一个短RNA的定向循环

Cathrin E Hansen¹, Danilo Springstubbe¹, Sabine Müller¹

¹University Hospital Schleswig-Holstein, Campus Lübeck, Germany.

Methods in molecular biology (Clifton, N.J.)

|February 21, 2024

概括

研究人员开发了一种高效的基于RNA的系统,用于控制循环RNA (circRNA) 合成. 这种方法可以在没有外部酶的情况下生产circRNA,提供一种新的可控制的循环化技术.

科学领域:

分子生物学分子生物学
在RNA治疗方面,RNA疗法.
生物化学生化学

背景情况:

产生特定长度和序列的循环RNA (circRNA) 的挑战阻碍了基础研究和功能分析.
目前用于circRNA合成的方法包括酶, ribozymatic 和基于拼接的方法,但定向循环化仍然有限.
有效和可控的circRNA合成对于推进基于RNA的治疗和诊断至关重要.

研究的目的:

提出一个可负担和高效的基于RNA的系统的原理证明,用于控制的circRNA合成.
开发一种生产具有生理学3",5"-二链接的circRNA的方法.
建立一个可控制的circRNA合成协议,独立于外部酶辅助.

主要方法:

设计一种毛式 ribozyme 变体,循环 ribozyme 3 (CRZ-3),具有较差的自我分裂活性.
设计一个激活剂-聚胺复合物,以促进CRZ-3的自我裂变.
使用CRZ-3系统对circRNA进行受控合成.

主要成果:

展示了一种基于RNA的新型系统,用于有效合成circRNA.
使用工程制造的 ribozyme 和一个激活器复合体,实现了受控的 circRNA 形成.
在不需要外部酶支持的情况下成功生成circRNA.

结论:

开发的系统为控制的circRNA合成提供了负担得起和高效的方法.
这种基于RNA的方法为现有的circRNA生产方法提供了可控制的替代方案.
这些发现为改进用于研究和治疗应用的定制circRNAs的生成铺平了道路.

关键词:

激活器的激活器是什么生物工程是生物工程.催化剂是一种催化剂.一个圆形的圆形圆形.循环化的循环化这是一个RNARNARNARNARNA.利博酶是一种 ribozyme.精子中的精子.

更多相关视频

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

相关概念视频

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...