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针对衰老的膜上皮细胞,使用工程化介质干细胞衍生外细胞囊泡来治疗肺纤维化

Yaoying Long¹, Bianlei Yang², Qian Lei³

¹Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

|February 21, 2024

概括

工程干细胞衍生的囊泡向肺纤维化中的衰老细胞. 这些CD38-ARM-MSC-EV恢复细胞功能,并显示出治疗肺纤维化的潜力.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

2型膜上皮细胞 (AEC2) 衰老驱动肺纤维化 (PF).
分化集群38 (CD38),一种消耗NAD+的酶,标志着衰老细胞,并且在PF AEC2中升高,呈现出治疗点.
介质干细胞 (MSC) 衍生的细胞外囊泡 (EV) 提供无细胞的抗衰老和抗纤维化疗法.

研究的目的:

为了设计MSC-EV,专门针对表达CD38.8的老化的AEC2.
评估修改后的MSC-EVs (CD38-ARM-MSC-EVs) 在缓解肺纤维化的治疗潜力.

主要方法:

使用lentiviral transfection构建的CD38抗原受体膜修饰的MSC-EVs (CD38-ARM-MSC-EVs) 进行了检测.
在工程电动汽车中评估了CD38表达和抗纤维素微RNA含量.
在试验室和老年小鼠模型中评估了衰老的AEC2的向,在腹膜内给药后.

主要成果:

改造的CD38-ARM-MSC-EVs表现出增强的CD38抗原受体表达和抗纤维菌miRNAs.
在体外和体内,CD38-ARM-MSC-EVs有效地准衰老的AEC2.
用CD38-ARM-MSC-EVs治疗恢复了NAD+水平,逆转了上皮质-介质酶过渡,使衰老细胞复原,并缓解了老年小鼠的肺纤维化.

结论:

CD38-ARM-MSC-EVs表现出对老化的AEC2s的特定向.
这种工程 EV 技术显示出开发新型治疗肺纤维化病剂的巨大潜力.

关键词:

CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 CD38 膜上皮细胞是膜上皮细胞.细胞外囊泡中的细胞外囊泡.介质细胞干细胞介质细胞干细胞肺部纤维化肺部纤维化衰老是一种老化.

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