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昆虫病原性伪单体可以与昆虫病原性线虫及其互惠细菌共享昆虫宿主

Maria Zwyssig¹, Anna Spescha¹, Tabea Patt¹

¹Plant Pathology, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH) Zurich, CH-8092 Zurich, Switzerland.

The ISME journal

|February 21, 2024

概括

将昆虫病原性伪单体 (EPPs) 与昆虫病原性线虫 (EPNs) 以及它们的互惠细菌 (NABs) 结合起来,显示出对生物害虫控制的前景. 这些微生物可以在昆虫宿主中共存,彼此受益,并支持线虫繁殖.

科学领域:

微生物生态学微生物生态学
生物控制生物控制
昆虫病理学昆虫病理学

背景情况:

对昆虫害虫的生物控制往往面临局限性.
昆虫病原性伪单体 (EPPs),昆虫病原性线虫 (EPNs) 和它们的互惠细菌 (NABs) 的联合应用是一个有前途的战略.
在宿主体内的这些微生物之间的物种间相互作用尚不清楚.

研究的目的:

为了研究EPP Pseudomonas蛋白质CHA0和NAB Xenorhabdus bovienii SM5.5.之间的细菌-细菌相互作用的动态.
评估这些微生物在日益复杂的实验系统中的共存和影响.
评估结合EPPs,EPNs和NABs用于昆虫害虫控制的潜力.

主要方法:

在体外细菌群体评估.
在体内注射血淋巴到Galleria mellonella幼虫中.
通过自然感染途径 (EPP的口服,NAB的线虫载体) 与EPN的应用.

主要成果:

在体外,Xenorhabdus bovienii SM5在高细胞密度下被Pseudomonas蛋白质CHA0所超越.
这两种细菌在Galleria mellonella幼虫中通过血淋巴注射和自然感染途径共存.
细菌的存在并没有对线虫的繁殖或后代的毒性产生负面影响;细菌的存在受益于细菌促进的进入.

结论:

昆虫病原性伪单体可以与昆虫病原性线虫及其相互性细菌共享昆虫宿主.
同存发生在没有对所涉及的虫病原体的繁殖或健康产生重大负面影响的情况下.
结合EPP,EPN和NAB的应用是生物昆虫害虫控制的可行和有前途的战略.

关键词:

跨物种的相互作用.生物控制生物控制这是一种共存,共存.共同感染是一种共感染.昆虫病原性线虫虫类的线虫.主机共享服务提供商.杀虫剂类的伪蒙.线虫是相互关联的细菌.

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