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巨细胞对病原体的早期反应需要对核粒的动态调节

Sikandar Azam¹, Kaitlyn S Armijo¹, Chi G Weindel¹

¹Department of Microbial Pathogenesis and Immunology, Texas A&M University, School of Medicine, Bryan, TX 77807.

Proceedings of the National Academy of Sciences of the United States of America

|February 21, 2024

概括

通过Neat1 lncRNA调节的核,控制巨细胞中的炎症基因表达. 它们的动态组装和拆卸对于对病原体产生有效的免疫反应至关重要.

科学领域:

细胞生物学细胞生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

巨细胞炎症基因表达需要精确的控制,以平衡病原体防御并防止自我损伤.
无膜有机体 (MLOs) 越来越多地被认为可以调节基因表达以应对细胞压力.
在基因调节中涉及的是Neat1 lncRNA周围形成的核,MLO.

研究的目的:

研究核和Neat1 lncRNA在调节巨细胞内天生的免疫反应中的作用.
阐明在天生的免疫激活过程中控制斑动态的机制.
为了确定对巨细胞抗微生物活性的斑功能障碍的功能后果.

主要方法:

研究了与先天激动剂刺激后,在小鼠巨细胞中研究了斑动力学 (聚合和分解).
研究了对护光镜维护和拆卸的分子要求,包括转录和MAPK信号传递.
利用Neat1淘汰赛 (KO) 巨细胞评估对基因表达和病原体控制的影响.

主要成果:

巨虫虫表现出快速的,依赖于刺激的聚合和分离.
抛光镜的动态取决于活性转录,MAPK信号传递和Neat1通过RNA外体的降解.
Neat1 KO巨体显示关键炎症基因的表达受损,以及控制沙门氏菌和膀性口腔炎病毒复制的能力降低.

结论:

核斑在协调巨细胞的先天免疫反应方面发挥着至关重要的作用.
磁粒的动态组装和拆卸对于及时和适当的炎症基因表达至关重要.
像一样的MLOs是核景观的关键调节者,能够有效地防御宿主对病原体的防御.

关键词:

在Neat1 lncRNA中.这是先天免疫力.没有膜的有机细胞.核RNA外基因组中的核RNA.

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