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从骨髓巨和下调ERK/p38信号通路中获得的D-alanine抑制了骨质细胞形成

Xiaochi Chang¹, Jing Deng², Fengyi Zhou³

¹Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China; Department of Stomatology, the Affiliated Hospital of Qingdao University, Qingdao, China.

Archives of oral biology

|February 21, 2024

概括

通过减少骨髓衍生巨细胞的增殖和增加其亡,D-alanine抑制骨质细胞的形成. 这一发现表明D-alanine.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
骨生物学骨生物学骨生物学

背景情况:

一种类型的脂质乙烯酸 (LTA),一个阳性细菌毒性因子,含有D-alanine.
对于D-alanine在传染性骨损伤中的作用尚不清楚.
骨质细胞是骨再吸收的关键细胞,并与各种骨疾病有关.

研究的目的:

为了研究D-alanine对骨质细胞增殖,亡和分化的影响.
探索D-alanine作为治疗骨恢复性疾病的治疗剂的潜力.

主要方法:

用D-alanine刺激小鼠骨髓衍生的巨细胞 (BMM).
用CCK-8和流式细胞计量来评估细胞增殖和细胞亡.
骨质细胞分化通过TRAP染色和基因/蛋白质表达分析 (NFATc1,c-Fos,Blimp) 来评估.
测量了MAPK信号通路 (p38,ERK1/2) 和可溶性Sema4D水平.

主要成果:

在剂量依赖的方式中,D-alanine显著抑制了BMM的扩散.
D-氨酸显著诱导了BMM细胞亡.
通过降低关键基因和蛋白质表达,d-alanine显著抑制了骨质细胞分化.
D-alanine 抑制了p38和ERK1/2 MAPK信号通路,并降低了可溶性Sema4D水平.

结论:

D-氨酸在抑制RANKL诱导的骨质结晶发生方面发挥着至关重要的作用.
德氨酸显示出作为治疗治疗骨复苏性疾病的治疗候选者的潜力.

关键词:

来自骨髓的巨细胞.D-阿兰氨酸的使用方法在MAPK信号通道上.骨质细胞生成 (Osteoclastogenesis) 是一个过程.这就是Sema4D的意义.

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