在Campylobacter jejuni从肝分离物中检测和分布集群定期间隔的短Palindromic重复 (CRISPRs)
Hung-Yueh Yeh1, Nelson A Cox1, Arthur Hinton1
1U.S. National Poultry Research Center, Agricultural Research Service, United States Department of Agriculture, 950 College Station Road, Athens, GA 30605-2720, USA.
Journal of food protection
|February 21, 2024
概括
大多数来自肝的Campylobacter jejuni分离物都含有CRISPR序列,通常与细菌菌体相同. 这一发现对于在家禽生产中开发有效的菌体为基础的坎皮洛巴克特控制策略至关重要.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 基因组学就是基因组学.
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是全球性食物传播疾病的主要原因,通常与家禽产品有关.
- 菌体治疗是减少家禽中坎皮洛巴克特菌污染的有希望的策略.
- 细菌的CRISPR-Cas系统可以对菌体产生抗性,这可能会阻碍菌体治疗的有效性.
研究的目的:
- 调查来自肝的Campylobacter jejuni分离物中CRISPR-Cas系统的流行率和特征.
- 在CRISPR数组中识别特定的间隔序列,这些序列可能会对细菌菌体产生抗性.
- 为了帮助制定有效的菌根控制措施,对家禽中的坎皮洛巴克特.
主要方法:
- 从肝样本中分离和识别Campylobacter jejuni.
- 基因组DNA提取和CRISPR1型序列的PCR放大.
- 桑格测序,CRISPRFinder分析DRs和间隔器,以及CRISPRTarget用于菌体同质识别.
主要成果:
- 178个Campylobacter jejuni分离物中的87%含有CRISPR序列,所有阳性分离物中都发现了一个常见的直接重复 (DR) 类型.
- 克里斯普尔位点的长度各不相同 (97-431 bp),并且包含1-6个间隔器,共计371个间隔器跨越51个独特的序列.
- 鉴定到的显著部分间隔序列显示出与坎皮洛巴克特菌DA10的同质性.
结论:
- 在来自家禽的Campylobacter jejuni中,CRISPR序列普遍存在,这表明可能存在菌素耐药性.
- 与细菌体DA10相同的已识别的间隔器为细菌防御机制提供了洞察力.
- 了解Campylobacter中的CRISPR-Cas对于优化家禽生产中菌体治疗策略至关重要.
关键词:
细菌菌体是一种细菌体.克里斯普尔是什么意思?克里斯普尔是什么意思?坎比洛巴克特 (Campylobacter jejuniuni) 是一个常见的菌类.肝是一种肝.聚类有规律间隔的短palindromic重复.更多相关视频
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