在暴露于二-3后,小鼠乳腺的分支形态发生

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Gonzalo Schierano-Marotti¹, Gabriela A Altamirano¹, Sofia Oddi²

¹Instituto de Salud y Ambiente del Litoral (ISAL, UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Cátedra de Patología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.

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概括

相-3 (BP3) 是一种常见的紫外线过器,但并没有改变小鼠的乳腺发育. 然而,BP3暴露影响了孕激素受体 (PR) 基因表达,表明对其他乳腺发育阶段的潜在影响.

科学领域:

内分泌学在内分泌学.
发展生物学发展生物学
毒理学毒理学毒理学

背景情况:

青春期乳腺分支形态发生是一个由激素调节的关键过程.
干扰内分泌的化学物质,如紫外线过器三 (BP3) 可以干扰这个过程.
了解BP3对乳腺发育的影响至关重要,因为它对人类的广泛暴露.

研究的目的:

调查子宫内和体外BP3暴露对雌性小鼠乳腺分支形态发生的影响.
评估BP3暴露后关键分子标记物和组织形态学的变化.

主要方法:

怀孕的小鼠在妊娠期间通过皮肤暴露于BP3.
哺乳动物有机体被培养并暴露在体外BP3.
对后代的乳腺进行了分析,以测量组织形态学,增殖和基因/蛋白质表达.
孕激素受体 (PR),WNT4,RANKL和安菲瑞古林的基因表达通过mRNA水平量化.

主要成果:

在子宫内,BP3暴露降低了孕激素受体 (PR) 和WNT4.4的mRNA水平.
在实验室中,BP3暴露降低了PR,RANKL和安菲瑞古林的mRNA水平.
没有观察到乳腺组织形态学,增殖或血清激素水平的显著变化.
乳腺分支的进展在体外没有受到BP3直接暴露的影响.

结论:

在研究的小鼠模型中,索-3 (BP3) 暴露不会损害乳腺分支形态发生.
BP3 影响孕激素受体 (PR) 和其下游介质的转录表达.
这些发现表明BP3对乳腺其他发育阶段的潜在影响,需要进一步调查.

关键词:

三维文化是3D文化.索芬-3的使用方法分支形态发生的分支形态发生.荷尔蒙的调节荷尔蒙的调节哺乳腺腺体哺乳腺体孕激素受体是什么? 孕激素受体是什么?

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