在豆中丧失性别的转录基准
M D Huguet1, S Robin1,2, S Hudaverdian1
1Institute for Genetics, Environment and Plant Protection, IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France.
BMC genomics
|February 21, 2024
概括
研究了豆虫失去性繁殖的遗传基础. 发现一个特定的840 kb区域及其基因会损害表观遗传和转录后规则,维持无性繁殖.
科学领域:
- 进化生物学 进化生物学
- 遗传学 是一个遗传学.
- 生殖生物学 生殖生物学
背景情况:
- 性生殖和无性生殖之间的过渡是常见的,但不太了解.
- 豆 (Acyrthosiphon pisum) 呈现繁殖多态性,具有循环和义务无性血统.
- 一个含有32个基因的840kb特定的基因组区域以前与这种繁殖多态性有关.
研究的目的:
- 为了确定对豆虫的性繁殖丧失负责的特定基因.
- 了解性丧失对控制性与无性胚胎生成的遗传程序的后果.
- 在对光周期线索的反应中,研究性和无性豆菌系之间的转录组差异.
主要方法:
- 性和强制性无性豆虫系的比较转录学.
- 分析重点是头部组织 (光周期检测) 和胚胎 (发育目标).
- 研究了对光周期缩短的反应中的差异性基因表达.
主要成果:
- 在840kb区域内,四个基因显示出两种基因系之间的差异性光周期反应 (一个在头部,三个在胚胎中).
- 性女性的胚胎发育涉及约1600个基因,其中微RNA (miRNA),PIWI相互作用RNA (piRNA) 和基因素修饰途径的过度代表性.
- 这些关键的发育基因位于富含可转移元素 (TE) 的基因组区域.
结论:
- 在840 kb区域内发现的遗传变异可能会损害无性血统中的表观遗传和转录后调节机制.
- 这些障碍维持了 pea aphids 强制性的无性生殖战略.
- 了解这些机制,可以了解真核生物生殖模式的演变.
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