增加的CO2固定使酵母中的3-基酸能够产生高碳产量的3-基酸
Ning Qin1, Lingyun Li1,2, Xiaozhen Wan1
1College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Nature communications
|February 21, 2024
概括
二氧化碳 (CO2) 固定增强生物基生产的3-基酸 (3-HP). 工程酵母实现了近乎理论上的产量,为生物烯酸和二氧化碳作为唯一的碳来源铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 生物基生产的成本竞争力依赖于高效的二氧化碳 (CO2) 固定.
- 由于二氧化碳同化方面的挑战,3-基酸 (3-HP) 的生产受到限制.
研究的目的:
- 为了证明二氧化碳固定如何能够实现理论上的产量生产3-HP.
- 为了克服二碳酸盐吸收和运输的局限性,以增强3HP生物合成.
- 为了高产量生产3-HP,生物烯酸的前体的工程酵母.
主要方法:
- 利用基因组规模的代谢模型来计算生产信封.
- 开发了增强二碳酸盐吸收的策略,包括识别Sul1作为载体.
- 通过域互换改造了马洛尼尔-CoA还原酶,并将Esbp6确定为潜在的出口商.
- 删除Uga1以防止3-HP降解.
主要成果:
- 确定了Sul1作为潜在的二碳酸盐运输商,Esbp6作为3HP出口商.
- 通过实施多种合理设计策略,成功设计了酵母菌株.
- 使用葡萄糖作为基质,实现了从0.14g/L到11.25g/L的3-HP产量的显著增加.
- 在与生物质形成并发的3HP生产中证明了接近最大的理论产量.
结论:
- 工程酵母菌株为生物烯酸的商业化提供了基础.
- 开发的二氧化碳固定策略适用于利用二氧化碳作为生物生产中唯一的碳来源.
- 这项工作突出了代谢工程在提高生物化学制造效率和可持续性方面的潜力.
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