Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Notch Signaling Reprograms Glial Lipid Metabolism to Promote Hypoxia Resistance.

bioRxiv : the preprint server for biology·2026

Same author

Multiscale metabolic mapping of lung tissue via coregistered mass spectrometry and nonlinear optical imaging.

Science advances·2026

Same author

3D Passive Cavitation Mapping (3D-PCM) with a Large-aperture Planar Array.

bioRxiv : the preprint server for biology·2026

Same author

Illuminating aging with multimodal optical metabolic imaging.

Science advances·2026

Same author

AMPK/ SIRT1 signaling pathway activation acts on PGC-1α/ PPARγ to alleviate sepsis-acquired weakness.

Cell death discovery·2026

Same author

Lysosome-derived methylated arginine is a signalling metabolite controlling the lipidome.

Nature cell biology·2026

Search research articles

相关实验视频

Updated: Jul 2, 2025

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

多分子高光谱PRM-SRS显微镜

Wenxu Zhang¹, Yajuan Li¹, Anthony A Fung¹

¹Shu Chien-Gene Lay Dept. of Bioengineering, University of California San Diego, La Jolla, CA, USA.

Nature communications

|February 21, 2024

概括

此摘要是机器生成的。

这项研究引入了一种新的超光谱成像方法,PRM-SRS显微镜,用于绘制细胞和组织中的脂质分布图. 它为研究衰老和疾病中的脂质提供了增强的化学特异性和分辨率.

更多相关视频

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

相关实验视频

Last Updated: Jul 2, 2025

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

科学领域:

生物化学生物化学
细胞生物学细胞生物学
医疗成像医学成像

背景情况:

脂质对生物过程至关重要,了解它们的空间分布和代谢动态是衰老和疾病研究的关键.
现有的成像技术在保护本地细胞环境,空间分辨率或区分脂质亚型方面存在局限性.

研究的目的:

开发和介绍一种用于高分辨率脂质分析的新型超光谱成像平台.
在脂质研究中克服传统成像方法的局限性.

主要方法:

开发一个高光谱成像平台,将惩罚性参考匹配 (PRM) 算法与刺激拉曼散射 (SRS) 显微镜 (PRM-SRS) 集成在一起.
应用PRM-SRS平台可视化和识别各种生物样本中的特定脂类亚型.

主要成果:

在人类脏中成功可视化和识别高密度脂蛋白颗粒.
在小鼠海马体颗粒细胞中检测高胆固醇与酸乙醇胺的比率.
在人类大脑组织中绘制sphingosine和cardiolipin的亚细胞分布图.

结论:

PRM-SRS平台提供了增强的化学特异性,亚细胞分辨率和快速的数据处理,用于脂类亚型分析.
这种方法在区分不同器官和物种的脂质亚型方面是有效的,在生物学和医学中推进了脂质研究.