在SINEUP非编码RNA介导的转录后基因调节中解密SINE重复元素的序列,结构和功能特征
Harshita Sharma1, Matthew N Z Valentine1, Naoko Toki1
1Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, 230-0045, Japan.
Nature communications
|February 21, 2024
概括
这项研究揭示了SINE RNA中保存的RNA结构动机对于SINEUP长非编码RNA来提高基因翻译至关重要. 这些结构与不同细胞区的蛋白质和核糖体RNA有动态相互作用.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 在RNA生物学,RNA生物学.
背景情况:
- RNA结构对基因调节至关重要,提供灵活性和功能多样性.
- 目前的in silico和in vitro方法难以完全捕捉细胞环境中的动态RNA结构.
- 含有SINE衍生的逆转移子的SINEUP长非编码RNAs在转录后调节基因表达.
研究的目的:
- 在SINEUPs中研究小鼠和人类SINERNA的序列,结构和功能特征.
- 阐明保存结构图案在SINEUP介导的翻译增强中的作用.
- 了解SINERNAs在细胞区内的动态结构变化和相互作用.
主要方法:
- 检测细胞内二次结构以分析功能性SINERNAs.
- 分析RNA-RNA相互作用,特别是与核糖体RNA的相互作用.
- 使用实验二次结构数据预测3DRNA架构.
- 对RNA结合蛋白 (RBP) 相互作用的研究.
主要成果:
- 功能性SINE RNA具有保存的短结构动机,这对于SINEUP诱导的翻译增强至关重要.
- SINE RNA结构在细胞核和细胞质之间表现出动态变化,与分区特定的RBP结合相关.
- 由SINE衍生的RNA与核糖体RNA直接相互作用,这表明一种翻译调节机制.
- 由于保留的相互作用特征,在进化上遥远的SINE转录RNA中观察到融合的功能角色.
结论:
- 在SINE RNA中保存的结构动图是SINEUP功能的关键驱动因素.
- 动态RNA结构和与RBPs和rRNA的分区特异性相互作用是SINEUP介导的基因调节的基础.
- 这项研究强调了RNA结构在跨进化上不同RNA的功能融合中的重要性.
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