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与微卫星标记器相比,单核酸多形态的有效性在摩洛哥马匹中用于亲属验证的评估

O Aminou¹, B Badaoui^2,3, M Machmoum¹

¹Veterinary Genetic Analysis Laboratory, Hassan II Agronomic and Veterinary Institute, Rabat, Morocco.

Animal genetics

|February 22, 2024

概括

单核酸多态 (SNP) 标志物显示出用于马匹亲属验证的高潜力,与短串联重复 (STR) 相比,提供更高的排除概率. 这项研究验证了SNP对马匹识别和保护工作的有效性.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
法医科学法医科学法医科学

背景情况:

国际动物遗传学学会 (ISAG) 建议转向单核酸多态性 (SNP) 标记物,以验证马的亲属关系.
目前的马匹亲属测试依赖于16个微卫星 (STR) 标记,不包括LEX3.
对STR进行SNP标记器性能评估对于验证这一转变至关重要.

研究的目的:

评估144个SNP基因定型小组用于验证马匹亲属关系的有效性.
为了比较SNP标记器与传统STR标记器的性能.
评估SNP在保护摩洛哥马匹品种方面的潜力.

主要方法:

通过使用144个SNP面板,对来自五种品种的1074匹马 (432只小马,414只母马,228只马) 进行基因定型.
基因参数的比较分析,包括预期和观察到的异构性,身份概率和SNP和STR之间的多态信息内容.
评估SNP标志物的一致性与Hardy-Weinberg平衡在多倍体.

主要成果:

在删除两个不一致的标记器后,最终确定了142个SNP的面板.
与STR相比,SNP表现出较低的预期平均值 (0.457) 和观察到的 (0.72) 异合性 (STR分别为0.76和0.72).
142个SNP的累计排除概率超过了99.99%,超过了STR (99.8%的父母之一,99.9%的父母).

结论:

142个SNP小组证明了对马匹亲属测试的高效性,符合ISAG的建议.
无线网络提供了一个强大的替代STR,特别是在排除概率方面.
SNP应用可以帮助保护摩洛哥马匹品种,尽管SNP面板的进一步完善是必要的.

关键词:

国家统一计划 (SNP) 是一个国家统一计划.排除,排除,排除的原因是什么?基因型定型是指基因型定型.马匹马匹马匹亲属关系亲属关系可能性概率概率的概率.测序的测序是指测序的测序.

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