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热应力促进了阿拉比多普西斯AGO1的相分离和与应力颗粒组件的结合

Aleksandar Blagojevic¹, Patricia Baldrich², Marlene Schiaffini¹

¹Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084 Strasbourg, France.

|February 22, 2024

概括

热应激会导致ARGONAUTE1 (AGO1) 蛋白在Arabidopsis thaliana的细胞凝结物中积聚. 这种局部化似乎是为了保护AGO1,而不是改变其在压力期间的基因沉默活动.

科学领域:

分子生物学分子生物学
植物科学植物科学
遗传学是一个遗传学.

背景情况:

在Arabidopsis thaliana中,ARGONAUTE1 (AGO1) 对于RNA沉默通路 (miRNA和siRNA) 是至关重要的.
AGO1在内质网膜中起作用,调解转化抑制,mRNA裂变和siRNA生物发生.
在压力条件下AGO1的作用,特别是热应力,尚未完全理解.

研究的目的:

为了研究热应激 (HS) 对AGO1蛋白位址和功能在Arabidopsis thaliana中的影响.
确定与其Poly-Q域相关的AGO1的相隔能力是否参与其对HS的反应.
阐明HS如何影响AGO1与小RNA的相互作用以及它在压力期间对基因表达调节中的作用.

主要方法:

在37°C的热应力下,分析AGO1蛋白在细胞凝结物中的积累和局部化.
显微镜观察AGO1与siRNA体和压力颗粒组件的同位化.
评估小RNA库,miRNA加载AGO1,以及HS后的目标mRNA裂变特征.
研究 AGO1 的 N-终端 Poly-Q 域在 HS 诱导的相分离中的作用.

主要成果:

热应激诱导AGO1蛋白在细胞凝聚物中积累,与siRNA体和应激颗粒共定位.
AGO1的类Poly-Q域足以进行相位分离,独立于SGS3.
HS对小RNA概况,AGO1负载和裂目标有适度的影响,这表明保护而不是功能损害.
在凝结物中AGO1的局部化似乎在热应激期间屏蔽了它的活动.

结论:

高温诱导了Arabidopsis thaliana中含有AGO1的细胞凝结物的形成.
由其Poly-Q域介导的AGO1的相分离是对热应激的关键反应.
凝结物形成可能对AGO1起着保护作用,在压力下保持其在RNA沉默中的功能.

关键词:

细胞生物学细胞生物学分子生物学分子生物学植物生物学植物生物学

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