UBE2L3抑制氧化应激调节的亡,以加速骨肉瘤的进展
Xiwu Zhao1, Guoqiang Shan2, Deguo Xing1
1Department of Traumatic Orthopedics, The Second Hospital of Shandong University, Jinan, 250033, China.
Recent patents on anti-cancer drug discovery
|February 22, 2024
概括
在骨髓瘤中,UBE2L3 (Ubiquitin-conjugating enzyme E2 L3) 的升高调节. 沉默UBE2L3会增加氧化应激,增强亡并延迟骨髓瘤的进展,提供一种潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞应激反应的应激反应
背景情况:
- 骨髓瘤是一种具有有限治疗选择的侵袭性骨瘤.
- 氧化应激与瘤发展有关,但其在骨髓瘤中的调节机制尚不清楚.
- UBE2L3在氧化应激和骨髓瘤进展中的作用需要进一步研究.
研究的目的:
- 阐明UBE2L3在促进骨髓瘤中氧化应激诱导的亡中的分子机制.
- 评估向UBE2L3用于骨髓瘤治疗的潜力.
主要方法:
- 在体外培养人类骨质母细胞和骨髓瘤细胞系.
- 用UBE2L3沉默等离子体 (si-UBE2L3) 或负对照 (si-NC) 进行传染.
- 检测细胞活力 (道),增殖 (克隆基),迁移/入侵 (特朗斯韦尔),亡 (流细胞计),ROS水平 (免疫光) 和与亡相关的蛋白质表达 (PCR,WB).
主要成果:
- 在骨髓瘤细胞中,UBE2L3mRNA水平明显高于正常骨质母细胞.
- UBE2L3的淘汰抑制了细胞活力,增殖,迁移和入侵,同时增强了细胞亡.
- 沉默UBE2L3增加了氧化应激,并通过上调RIP1,RIP3和MLKL酸化来促进亡.
结论:
- 在骨髓瘤中,UBE2L3的升高调节有助于瘤的进展.
- 制UBE2L3会增加氧化应激和亡,从而抑制骨髓瘤的进展.
- 准UBE2L3代表了骨髓瘤的一个有前途的治疗策略.
关键词:
骨质肉瘤骨质肉瘤是什么这就是ROSOS ROS.在UBE2L3L3中使用.亡 (apoptosis) 是一种死亡的过程.尸体亡 (necroptosis) 是一种致死症.氧化应激是一种氧化应激.更多相关视频
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