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细胞铁耗尽通过限制大脑Per1表达在小鼠中增强行为节奏

Qiong Wu^1,2, Qiuyang Ren¹, Xin Wang¹

¹Laboratory of Molecular Iron Metabolism, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, Hebei Collaborative Innovation Center for Eco-Environment, Hebei Research Center of the Basic Discipline of Cell Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

CNS neuroscience & therapeutics

|February 22, 2024

概括

改变大脑铁水平会影响昼夜节律,影响PER1的表达,影响运动活动和时钟基因功能. 这项研究表明大脑的铁调节作为与年龄相关的昼夜干扰的新疗法目标.

科学领域:

神经科学是一个神经科学.
时间生物学时间生物学
铁的新陈代谢铁的新陈代谢

背景情况:

循环节律障碍与衰老和神经退行性疾病有关,通常涉及大脑铁积累.
大脑铁在调节生物节律中的确切作用仍然在很大程度上未被探索.

研究的目的:

为了研究大脑铁水平如何影响小鼠的自发发动机活动.
阐明连接大脑铁和昼夜节律调节的潜在分子机制.

主要方法:

利用条件淘汰赛小鼠模型 (内皮细胞中的Fpn1) 来操纵大脑的铁水平.
检查了运动运动活动,时钟基因表达 (时钟,Bmal1,PER1) 和血清黑激素.
研究铁对PER1表达的人类质瘤 (U251) 和小鼠大脑星细胞 (MA-c) 细胞系 in vitro 的影响.

主要成果:

大脑缺铁增强了运动活动和改变了时钟基因表达,降低了PER1.
在APP/PS1小鼠中,脑铁水平升高抑制了节律活动,并增加了血清黑激素.
铁含量调节了两个细胞系中的PER1表达,缺铁减少了PER1,而铁补充增加了它.

结论:

改变大脑铁水平通过调节PER1表达和调节分子昼夜时钟,直接影响昼夜节律.
脑铁调节成为与年龄相关的昼夜节律障碍的潜在治疗点.

关键词:

每一个1个1个衰老的衰老衰老的衰老昼夜节律昼夜节律时钟基因的时间基因铁是铁,铁是铁,铁是铁.

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