循环的无细胞DNA在绝经后高于绝经前和绝经前妇女
Teagan Fisher1, Elyse Powell, Nicole L Yuwono
1From the Gynaecological Cancer Research Group, Adult Cancer Program, School of Clinical Medicine University of New South Wales, Sydney, Australia.
Menopause (New York, N.Y.)
|February 22, 2024
概括
与绝经前妇女相比,绝经后妇女的血循环无细胞DNA (cirDNA) 度显著更高. 这些发现对于开发精确的cirDNA液体活检测试来说至关重要,特别是对于与年龄相关的疾病.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 在瘤学瘤学.
背景情况:
- 循环无细胞DNA (cirDNA) 越来越多地用于疾病查的液体活检.
- 影响cirDNA水平的生物学因素需要对临床测试准确性的彻底调查.
- 更年期对cirDNA的影响尚未得到广泛研究.
研究的目的:
- 在健康的绝经前和绝经后妇女中比较血cirDNA度和DNA片段完整性.
- 为了研究更年期状态和cirDNA特征之间的关系.
主要方法:
- 在47名健康的女性志愿者 (26名绝经前,21名绝经后) 中,使用定量聚合酶链反应 (qPCR) 提取和定量化了血cirDNA.
- 使用ALU重复序列 (115bp和247bp) 测量cirDNA度.
- 评估DNA片段的完整性是长 (247 bp) 与短 (115 bp) 片段的比例.
主要成果:
- 绝经后的女性表现出显著更高的血环DNA度 (P < 0.0001) 和更高的长链环DNA碎片度 (P = 0.0033).
- 在更年期前和更年期后的组之间没有观察到cirDNA完整性比的显著差异 (P = 0.1788).
结论:
- 绝经后妇女的血cirDNA度较高.
- 这些发现对cirDNA液体活检的开发和应用有重大影响.
- 了解这些差异对于癌症等疾病尤为重要,癌症主要在绝经后的个体中被诊断出来.
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