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来自Achromobacter denitrificans的阿斯巴酸脱酶的表征和应用

Zifeng Wang¹, Wenjing Liu¹, Yi Yan¹

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

Applied biochemistry and biotechnology

|February 22, 2024

概括

来自Achromobacter denitrificans的新酸脱酶 (ASPDH) 酶可以有效地将2 - 基托黄油酸转化为L-2-氨基黄油酸 (L-2-ABA). 这种生物催化剂系统使用合酶实现高产量的辅因子再生.

科学领域:

生物化学生物化学
酶学是一种酶学.
微生物生物技术微生物生物技术

背景情况:

在Achromobacter denitrificans中发现了一种编码阿斯巴酸脱酶 (ASPDH) 的新基因.
ASPDH对氧化酸和2 - 黄酸表现出降解活性,具有严格的NADH依赖.

研究的目的:

为了描述新型的阿斯巴酸脱酶 (ASPDH) 酶.
开发一种用于生产L-2-氨基黄油酸 (L-2-ABA) 的生物催化工艺.

主要方法:

ASPDH的酶特性,包括动力参数的确定 (K_m,V_max,K_cat).
研究金属离子 (EDTA,Cu2+,Ca2+,Fe2) 对酶活性的影响.
使用葡萄糖脱酶 (GDH) 和ASPDH用于辅因子再生的合全细胞生物催化剂系统的开发.

主要成果:

ASPDH 证明了氧化酸和2 - 黄酸的特定动态参数.
酶活性是由特定的金属离子调节的,Ca2+和Fe2+增强了活性,EDTA/Cu2+抑制了它.
结合的GDH-ASPDH系统有效地将2-脂酸转化为L-2-ABA,产量为97.2%.

结论:

新型ASPDH酶是L-2-ABA合成的有希望的生物催化剂.
开发的全细胞生物催化剂系统为生产L-2-ABA提供了一种高效和高产的方法.
这项研究扩大了氨基酸生产的酶化工具箱.

关键词:

2 - 基托黄油酸是什么?亚斯巴酸脱酶的使用方法 L-2-阿米诺黄油酸是什么?全细胞生物催化剂全细胞生物催化剂

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