Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Probing the Structure and Dynamics of Telomeric G-Quadruplexes with Thienoguanosine, a Fluorescent Analogue of Guanosine.

ACS chemical biology·2026
Same author

Association between infection and mortality in acute heart failure in the emergency department: a cohort study.

European journal of emergency medicine : official journal of the European Society for Emergency Medicine·2026
Same author

Quantitative fluorescence imaging of the impact of the nucleocapsid domain deletion on the dynamics of HIV-1 Gag assembly.

Methods and applications in fluorescence·2026
Same author

Occupational Asthma Without Nonspecific Bronchial Hyperresponsiveness.

The journal of allergy and clinical immunology. In practice·2026
Same author

Growing the family of fluorescence.

Methods and applications in fluorescence·2026
Same author

Harmonization and standardization of personalized oncology care within the German network for personalized medicine (DNPM): methods of a controlled observational study employing an adapted stepped-wedge design.

Frontiers in health services·2026

相关实验视频

Updated: Jul 2, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K

有针对性的单个粒子跟踪与升级纳米粒子.

Oleksii Dukhno1, Srijayee Ghosh1, Vanille Greiner1

  • 1Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, Strasbourg 67000, France.

ACS applied materials & interfaces
|February 22, 2024
PubMed
概括
此摘要是机器生成的。

升级纳米粒子 (UCNPs) 为生物分子在活细胞中的单颗粒追踪 (SPT) 提供了卓越的性能. 这些UCNP标签克服了传统染料和量子点的局限性,使细胞动态更清晰地可视化.

关键词:
高亲和度的IgE受体纳米颗粒是一种纳米粒子.单颗粒追踪器是一个单颗粒追踪器.单分子显微镜的显微镜.上升转换是一种上升转换.

更多相关视频

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.2K

相关实验视频

Last Updated: Jul 2, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.2K

科学领域:

  • 生物物理学的生物物理.
  • 纳米技术 纳米技术
  • 细胞生物学 细胞生物学

背景情况:

  • 单颗粒跟踪 (SPT) 可实时可视化生物分子运动.
  • 现有的标签,如光染料和量子点有局限性 (光稳定性,闪).
  • 向上转换纳米粒子 (UCNPs) 作为先进的SPT标签显示出希望.

研究的目的:

  • 使用UCNPs展示目标的SPT.
  • 合成和描述UCNP用于细胞跟踪.
  • 将UCNP SPT性能与传统标签进行比较.

主要方法:

  • 合成的30纳米化UCNP,涂有PEGylated聚合物.
  • 功能化的UCNP与免疫球蛋白E (IgE) 通过生物-链维丁.
  • 在使用UCNP-IgE标签的RBL-2H3巨细胞上追踪IgE受体 (FcεRI).
  • 直接比较UCNP-SPT与有机染料 (AlexaFluor647) 和量子点 (QD655).

主要成果:

  • 开发出水中可分散,明亮和均的UCNP.
  • 在活体巨细胞上成功跟踪FcεRI.
  • UCNP-SPT提供了卓越的光稳定性和持续发光.
  • 与传统标签不同,UCNP的性能不受标签光物理的限制.

结论:

  • UCNPs是活细胞中针对性SPT的高效标签.
  • 基于UCNP的SPT克服了传统标签的光物理限制.
  • 这一进步允许更准确地研究细胞动力学.