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有针对性的单个粒子跟踪与升级纳米粒子

Oleksii Dukhno¹, Srijayee Ghosh¹, Vanille Greiner¹

¹Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, Strasbourg 67000, France.

ACS applied materials & interfaces

|February 22, 2024

概括

升级纳米粒子 (UCNPs) 为生物分子在活细胞中的单颗粒追踪 (SPT) 提供了卓越的性能. 这些UCNP标签克服了传统染料和量子点的局限性,使细胞动态更清晰地可视化.

科学领域:

生物物理学的生物物理.
纳米技术纳米技术
细胞生物学细胞生物学

背景情况:

单颗粒跟踪 (SPT) 可实时可视化生物分子运动.
现有的标签,如光染料和量子点有局限性 (光稳定性,闪).
向上转换纳米粒子 (UCNPs) 作为先进的SPT标签显示出希望.

研究的目的:

使用UCNPs展示目标的SPT.
合成和描述UCNP用于细胞跟踪.
将UCNP SPT性能与传统标签进行比较.

主要方法:

合成的30纳米化UCNP,涂有PEGylated聚合物.
功能化的UCNP与免疫球蛋白E (IgE) 通过生物-链维丁.
在使用UCNP-IgE标签的RBL-2H3巨细胞上追踪IgE受体 (FcεRI).
直接比较UCNP-SPT与有机染料 (AlexaFluor647) 和量子点 (QD655).

主要成果:

开发出水中可分散,明亮和均的UCNP.
在活体巨细胞上成功跟踪FcεRI.
UCNP-SPT提供了卓越的光稳定性和持续发光.
与传统标签不同,UCNP的性能不受标签光物理的限制.

结论:

UCNPs是活细胞中针对性SPT的高效标签.
基于UCNP的SPT克服了传统标签的光物理限制.
这一进步允许更准确地研究细胞动力学.

关键词:

高亲和度的IgE受体纳米颗粒是一种纳米粒子.单颗粒追踪器是一个单颗粒追踪器.单分子显微镜的显微镜.上升转换是一种上升转换.

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