在Saccharomyces cerevisiae中优化从纳灵宁中提的生物合成
Yuyang Pan1, Zhibo Yan1, Songlyu Xue1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Journal of agricultural and food chemistry
|February 22, 2024
概括
使用基因酵母表达关键酶的工程酵母,提高了二甲 (DHQ) 产量. 一种新型的F3H突变使得DHQ从宁素 (NAR) 的产量增加了1.67倍,达到71.26%的高转化率.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 酶工程是什么? 酶工程是什么?
背景情况:
- 乙二 (DHQ) 具有显著的生理益处和工业应用.
- 由于中间积累,DHQ生物合成受到阻碍,限制了生产效率.
研究的目的:
- 通过酵母中的代谢工程来提高DHQ生产效率.
- 开发一种优化的flavanone-3-hydroxylase (F3H),以提高DHQ产量.
主要方法:
- 整合了F3H,黄-3'-基酶和细胞染色体P450减少酶的基因.
- 采用计算机辅助的蛋白质设计来创建一个最佳的F3H突变 (P221A).
- 优化了酶反应条件和培养基,用于从纳林根因 (NAR) 合成DHQ.
主要成果:
- 与对照组相比,F3H突变P221A增加了NAR的DHQ产量1.67倍.
- 从800 mg/L的NAR中获得了637.29 ± 20.35 mg/L的DHQ度.
- 从NAR合成DHQ达到71.26%的高转化率.
结论:
- 工程酵母菌株和优化的F3H显示了工业DHQ生产的巨大潜力.
- 开发的方法代表了从NAR.合成DHQ的高效途径.
- 这项研究提供了一个有希望的策略,以克服DHQ生物合成的局限性.
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