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Updated: Jul 2, 2025

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
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扰乱衍射方法解决了构成交换机,从而促进了两步酶机制

Jack B Greisman1, Kevin M Dalton1, Dennis E Brookner1

  • 1Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138.

Proceedings of the National Academy of Sciences of the United States of America
|February 22, 2024
PubMed
概括

蛋白质动力学对于酶催化是至关重要的. 这项研究揭示了埃舍里希亚大肠杆菌二叶酸减少酶 (DHFR) 如何利用结构动力学来促进质子和化物转移,提高催化效率.

关键词:
在 DHFR 中使用 DHFR.在X射线晶体学.亚洛斯特菌是什么意思?酶催化酶的催化作用蛋白质动力学 蛋白质动力学

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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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