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Jian Cui¹, Linwei Li¹, Shuang Wei¹

¹Institute of Cytology and Genetics, The Hengyang Key Laboratory of Cellular Stress Biology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, The First Affiliated Hospital, Institute of Cardiovascular Disease, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.

Ecotoxicology and environmental safety

|February 22, 2024

概括

低剂量的电离辐射会损害细胞,并降低谷氨S转移酶Pi (GSTP1) 的表达. 过度表达GSTP1部分逆转这些有害影响,突出其在辐射损伤中的保护作用.

科学领域:

辐射生物学辐射生物学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

低剂量的电离辐射对辐射工作者构成风险.
了解细胞对低剂量辐射的反应至关重要.
谷氨S转移酶Pi (GSTP1) 在辐射损伤中的作用需要研究.

研究的目的:

研究GSTP1在低剂量电离辐射损伤中的作用.
为了澄清电离辐射对细胞生物活动的影响.
分析暴露于辐射的个体和细胞中的GSTP1表达.

主要方法:

定量PCR (qPCR) 检测GSTP1mRNA的表达.
将GM12878细胞暴露于低剂量的马辐射 (14μGy/h).
流细胞计,MTS测定和细胞形态分析以评估细胞效应.
基因甲基化分析和相关信号分子的评估.

主要成果:

与健康对照组相比,在辐射工作者中观察到GSTP1mRNA表达的减少.
低剂量的电离辐射诱导了细胞形态的变化,抑制了增殖,延迟了细胞周期,并在GM12878细胞中增加了细胞亡.
过度表达GSTP1部分逆转了辐射诱导的细胞损伤.
低剂量的电离辐射增加了GSTP1基因甲基化,降低了其表达,并影响了相关的信号通路 (Nrf2,JNK).

结论:

低剂量的电离辐射会导致细胞损伤,影响细胞增殖,细胞循环和细胞亡.
GSTP1在调节细胞对低剂量电离辐射反应方面发挥着重要作用.
GSTP1调解低剂量电离辐射对Nrf2和JNK等信号分子的影响.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.细胞循环的细胞周期这是一个GSTP1GSTP1.电离辐射辐射的电离辐射.甲基化甲基化甲基化

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