UBE2J1通过STAT3/IRF1信号通路促进ALV-A前病毒DNA合成
Xingming Wang1, Shiling Zheng1, Chun Fang1
1School of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China.
Veterinary microbiology
|February 22, 2024
概括
E2J1 (UBE2J1) 通过增强前病毒性DNA合成和通过STAT3/IRF1途径抑制干扰素产生,促进禽类白血病病毒的复制.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 泛素结合酶E2J1 (UBE2J1) 参与哺乳动物的泛化和RNA病毒复制.
- UBE2J1在病毒复制和免疫方面的特定生物功能在很大程度上仍未被描述.
研究的目的:
- 为了研究UBE2J1在禽类白血病病毒 (ALV-A) 复制中的作用.
- 阐明UBE2J1影响病毒复制和宿主抗病毒反应的分子机制.
主要方法:
- 克隆UBE2J1和构建过度表达和shRNA敲击等离子体.
- 在UBE2J1调制下,在胚胎纤维细胞 (DF-1细胞) 中评估ALV-A复制.
- 对病毒复制阶段的分析,包括吸附,入侵,前病毒DNA合成和病毒粒子释放.
- 研究UBE2J1对STAT3/IRF1信号通路和干扰素产生的影响.
- 在UBE2J1.1.中的潜在酸化位点 (Ser184) 的位点定向突变发生.
主要成果:
- UBE2J1的过度表达显著促进了ALV-A复制,而敲击抑制了它.
- UBE2J1主要通过促进前病毒DNA合成来增强ALV-A复制.
- 发现UBE2J1通过抑制STAT3/IRF1通路来抑制干扰素的产生.
- 将Ser184转化为Gly的突变表明它作为UBE2J1功能关键酸化部位的作用.
- UBE2J1通过抑制STAT3/IRF1抗病毒通路,促进细胞中的ALV-A复制.
结论:
- UBE2J1在促进ALV-A复制方面发挥着至关重要的作用.
- UBE2J1通过增强的前病毒DNA合成和通过STAT3/IRF1途径抑制宿主的干扰素反应,促进病毒复制.
- 这项研究突出了与乌比奎丁相关的蛋白质在抗病毒免疫力中的重要性,并为控制禽类病毒感染提供了潜在的点.
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