单分子追踪揭示了通用转录因子NusG的功能分配,体内相互作用和空间组织
Hafez El Sayyed1, Oliver J Pambos1, Mathew Stracy2
1Gene Machines Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, UK; Kavli Institute of Nanoscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, Oxford, UK.
Molecular cell
|February 22, 2024
概括
细菌蛋白NusG主要与RNA聚合酶和核糖体结合,指导其在转录中的功能. 这项研究揭示了NusG在活体大肠杆菌细胞内的抗终结和转录-翻译合中的关键作用.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- NusG 是一个关键的转录因子,参与多个RNA聚合酶功能,包括暂停,终止和将转录与翻译合.
- 尽管已知NusG的作用,但其精确的体内本地化和功能分布在很大程度上仍未确定.
研究的目的:
- 阐明活体中NusG蛋白在活体大肠杆菌细胞中的功能分配和空间分布.
- 了解NusG如何整合其在转录监管中的多种角色.
主要方法:
- 单分子追踪和超分辨率成像在活的大肠杆菌.
- 对 NusG 种群及其扩散动态的分析.
- 使用抗生素治疗和删除突变物来探测功能.
主要成果:
- NusG主要存在于具有RNA聚合酶延长复合体的染色体相关群体中,以及与30S核糖体子单元复合的群体中.
- 一小部分NusG表现出快速扩散,这表明暂时的,非特异性的DNA相互作用.
- 染色体关联的NusG对于转录凝聚体内的rRNA操作子反终结和转录-翻译合至关重要.
结论:
- NusG利用不同的种群和动态行为来履行其在基因表达中的多方面的作用.
- 30S核糖体子单元作为NusG传递到转录延长部位的指南.
- 在体内成像提供了一种强大的方法来剖析像NusG这样的多功能分子机器的功能.
相关概念视频
01:53Bacterial Transcription
28.2K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.2K
02:11Reporter Genes
11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K