氨酸及其代谢物通过调节线粒体热生成和动力学来促进白色脂肪组织的增生
Eleonora Cremonini1, Leane M E Da Silva1, Cecilia Rodriguez Lanzi2
1Department of Nutrition, University of California, Davis, CA, USA; Department of Environmental Toxicology, University of California, Davis, CA, USA.
Biochemical pharmacology
|February 22, 2024
概括
安托西亚宁 (AC) 减轻高脂肪饮食对线粒体的影响. 补充AC可以改善白色脂肪组织中的线粒体生物生成,动力学和热生成,可能通过调节关键信号通路.
科学领域:
- 线粒体生物学 线粒体生物学
- 营养生物化学 营养生物化学
- 脂肪组织的新陈代谢
背景情况:
- 高脂肪饮食 (HFD) 的消费会破坏线粒体功能和动态,特别是在白色脂肪组织 (sWAT) 中.
- 氨酸 (AC) 之前已经显示出降低HFD诱导的体重增加和脂肪积累的潜力.
研究的目的:
- 为了研究安东基氨酸 (AC) 对抗小鼠sWAT.在线粒体动力学,生物生成和热生成中HFD诱导的变化的能力.
- 阐明特定的安托西亚宁 (?? 素-3-O-葡萄糖化物和素-3-O-葡萄糖化物) 和它们的肠道代谢物对脂肪细胞中的线粒体功能的作用机制.
主要方法:
- 给小鼠提供了控制或HFD饮食,有或没有AC补充剂.
- 在sWAT中分析了线粒体结构,生物发生蛋白 (PPARγ,PRDM16,PGC-1α),热发生标记物 (UCP-1),动态蛋白 (Drp-1,OPA1,MNF-2,Fis-1) 和线粒细胞衰变标记物 (BNIP3L/NIX).
- 用棕酸盐治疗的3T3-L1脂肪细胞被用来研究特定的安托西亚宁 (C3G,D3G) 和其代谢物 (PCA,HB,GA) 对信号通路 (cAMP,PKA,AMPK,SIRT-1) 和基因表达的影响.
主要成果:
- 消费HFD导致结构改变的sWAT线粒体较少,生物发生和热发生蛋白质减少,并破坏了线粒体动力学和线粒体衰变.
- 补充AC可以防止这些HFD诱导的线粒体变化,并降低AMPK酸化.
- 特定的氨酸 (C3G,D3G) 和它们的代谢物调节了信号通路中的棕酸盐诱导的变化,并阻止了关键线粒体生物发生和热发生基因表达的减少.
结论:
- 氨酸,特别是氨酸和氨酸,可以减轻白色脂肪组织中HFD诱导的线粒体功能障碍.
- 补充AC可促进sWAT线粒体生成,并改善线粒体动力学.
- 这些效应部分通过PKA,AMPK和SIRT-1信号通路的调节来调节,这表明AC在与肥胖相关的代谢障碍中的治疗潜力.
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