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Updated: Apr 10, 2026

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调节来自极端爱好者Bacillus ligniniphilus L1的瓦尼林脱酶酶的pH概况,通过计算引导的位点导向突变发生

Lingxia Xu¹, Muhammad Zohaib Nawaz¹, Hafiz Rameez Khalid¹

¹Biofuels Institute, School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; International Joint Laboratory on Synthetic Biology and Biomass Biorefinery, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, PR China.

International journal of biological macromolecules

|February 22, 2024

概括

香脱酶 (VDH) 酶工程增强了香酸的生产. 局部定向突变发生改善了VDH性耐受性和生物炼油应用中的活性.

科学领域:

生物催化和酶工程生物催化和酶工程
工业生物技术工业生物技术
蛋白质工程是一种蛋白质工程.

背景情况:

香脱酶 (VDH) 对于从香中产生香酸至关重要.
目前的VDH需要提高性耐受性和生物炼油工艺的效率.

研究的目的:

从Bacillus ligniniphilus L1中设计VDH,以提高性耐受性和活性.
为了确定在工业条件下增强酶性能的特定突变.

主要方法:

计算机引导的位点定向突变发生,准表面和活性位点残留物.
基于计算分析的20种残留物的选择和突变.
突变酶活性和最佳pH值的表征.

主要成果:

突变I132R和T235E的最佳pH转移到9和8.6,活性分别增加了2.82和2.95倍.
一个双重突变 (I132R/T235E) 呈现了pH最佳转移到9和74.91%的酶活性增加.
设计的VDH在性pH下显著提高了性能.

结论:

双重突变的VDH (I132R/T235E) 在工业应用方面显示出有前途.
通过位点定向突变发生的酶工程对于优化生物炼油厂的VDH是有效的.
这项研究提供了一个强大的VDH变体,用于高效的酸生产.

关键词:

活动站点活动站点表面电荷是指表面电荷.香酸是一种酸.瓦尼林脱酶的使用方法在pH值转移的过程中,pH值发生了变化.

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