[医院废水中的菌体生态分布的系统分析]
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
|February 22, 2024
概括
从医院的废水中分离出细菌菌体,向多种耐药细菌. 格拉姆阴性病原体显示出较高的菌体隔离率,与它们在废水中的丰富程度相关,与格拉姆阳性细菌不同.
科学领域:
- 微生物学和病毒学
- 环境科学 环境科学
- 传染性疾病 传染性疾病
背景情况:
- 菌体治疗是对抗多药耐药细菌感染的重要策略.
- 医院的废水是隔离治疗性菌的潜在来源.
- 缺乏关于菌体在医院废水中的分布的系统研究.
研究的目的:
- 为了分离菌体,准常见的临床病原性细菌.
- 评估病菌体在医院废水中的生态分布.
- 为隔离和应用对抗耐药细菌的菌体提供参考.
主要方法:
- 采用了横截面研究设计.
- 来自Qilu医院的125种致病细菌菌株被用作宿主.
- 使用双层板三明治方法分离细菌菌体;通过mNGS测序分析细菌分布.
主要成果:
- 隔离了56种菌株,目标是六种病原体:宝曼菌,肺炎菌,大肠杆菌,金黄色葡萄球菌,Pseudomonas aeruginosa和Stenotrophomonas maltophilia.
- 所有向的病原体都表现出不同程度的耐药性,其中A. baumannii,大肠杆菌和P. aeruginosa的高率.
- 菌体隔离率各不相同,大肠杆菌 (80%) 和S. maltophilia (75%) 显示高率;mNGS显示废水中高丰度的格拉姆阴性细菌.
结论:
- 常见的格拉姆阴性临床病原体的细菌体可以有效地从医院废水中分离出来.
- 针对格拉姆阳性细菌的菌体的隔离率较低,只发现了金黄色菌体.
- 污水中格兰氏阴性病原体的分布与它们相应的菌体的生态分布呈正相关.
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