在超的环境下,Labeo rohita的转录组中的lncRNA-miRNA-mRNA网络
Nitin Shukla1, Vemula Harshini1, Ishan Raval1
1Gujarat Biotechnology Research Centre, Sector 11, Gandhinagar, Gujarat, India.
Scientific data
|February 22, 2024
概括
这项研究揭示了度的增加如何影响Labeo rohita脏中的基因表达. 它识别了差异表达的长非编码RNA (lncRNA) 和微RNA (miRNA),在盐度压力下发现复杂的RNA相互作用.
科学领域:
- * 分子生物学 * 分子生物学
- * 水生毒理学 * 水生毒理学
- * 基因组学 是一个学科.
背景情况:
- * 淡水鱼在适应不同盐度时面临生理上的挑战.
- *了解盐度耐受性的分子机制对于水产养殖和保护至关重要.
- *鱼中的基因表达是环境应激反应的关键指标.
研究的目的:
- * 在逐渐增加的盐度度下研究Labeo rohita的脏转录组.
- * 为了识别不同表达的长非编码RNAs (lncRNAs) 和microRNAs (miRNAs),以应对盐度压力.
- * 构建竞争的内源RNA (ceRNA) 网络,以阐明不同盐度下的RNA相互作用.
主要方法:
- *使用高通量测序来生成10.25 Gbps的转录组数据.
- * 生物信息学工具 (FEELnc,CPC2,BLASTn) 用于 lncRNA 和 miRNA 的识别.
- *分析了差异表达的RNA,并构建了ceRNA网络.
主要成果:
- * 在盐度处理 (2,4,6和8 ppt) 中发现了大量的差异表达的lncRNA和miRNA.
- *差异表达的lncRNA的数量在99~269个之间,miRNA的数量在99~124个之间.
- * 构建了两个ceRNA网络 (A*和B*),在不同的盐度水平上显示了不同数量的lncRNA-miRNA-mRNA相互作用,其中8ppt组显示了最多的对.
结论:
- *盐度增加显著改变了Labeo rohita脏中的lncRNAs和miRNAs的表达.
- * 构建的ceRNA网络突出显示了复杂的调节相互作用,以应对盐度压力.
- *这些发现为淡水鱼的盐度适应的分子基础提供了洞察力.
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