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离心力的作用对骨髓单核细胞的隔离,使用密度梯度离心法

Tianyi Gu¹, Kongying Li², Xiaoyu Zhang³

¹The Second Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 33 Ba-Da-Chu Road, Shi-Jing-Shan District, Beijing, 100144, China.

Aesthetic plastic surgery

|February 22, 2024

概括

为骨髓单核细胞 (BMMNCs) 优化Ficoll离心是骨再生的关键. 300g的力量产生了最多的干细胞,证明优于200g,可与治疗应用中的400g相比.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

骨髓单核细胞 (BMMNCs) 在骨再生疗法方面表现有前景.
目前使用Ficoll密度梯度离心的隔离方法具有低于最佳的离心力.
优化BMMNC隔离对于提高治疗疗效至关重要.

研究的目的:

为了确定Ficoll密度梯度离心骨髓 (BM) 的最佳离心力.
为了最大限度地提高BMMNC的干细胞/祖细胞含量,用于再生应用.
评估离心力对BMMNC产量和质量的影响.

主要方法:

来自九只小猪的骨髓使用Ficoll密度梯度离心在200g,300g和400g时进行处理.
分析了残留红细胞 (RBC) 水平,核细胞计数,活力,亡和活性氧物种 (ROS).
评估了CD90表达,殖民地形成单元 (CFU-f) 和隔离的介质干细胞 (BMSCs) 的体外分化潜力.

主要成果:

300克产生的CD90+BMSC和BMSC-CFU-f显著高于200克,效率与400克相似.
200克导致红细胞污染最高,BMSC-CFU-f最低,可能阻碍细胞疗法.
在测试的力量之间没有观察到BMSC形态,增殖,表型或差异化潜力的显著差异.

结论:

300g代表了一个最佳的离心力来隔离BMMNCs通过Ficoll密度梯度离心用于骨再生疗法.
这种力量平衡了细胞产量和质量,这对于有效的BMMNC治疗至关重要.
进一步优化隔离协议可以提高BMMNCs的临床应用.

关键词:

骨髓单核细胞是骨髓中的单核细胞.骨髓衍生中酶体干细胞是骨髓衍生中酶体干细胞.离心力是一种离心力.密度梯度离心法离心法密度梯度离心法

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