相关实验视频

Updated: Jul 2, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K

聚合酶循环的应用,用于通过活细胞成像对新生转录的量化.

Olivia Kindongo1, Guillaume Lieb1, Benjamin Skaggs1

  • 1Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.

Yeast (Chichester, England)
|February 23, 2024
PubMed
概括

由于聚合酶循环,基因长度不会影响转录强度. 较短的基因可能通过这种机制产生更多的信使RNA (mRNA),受促进体和细胞条件的影响.

关键词:
在 MAPK 信号通道上.基因循环的基因循环.菌体外层蛋白质是一种菌体外层蛋白质.压力反应应对压力的反应.转录的动态 转录的动态

更多相关视频

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

9.1K
Measuring the Kinetics of mRNA Transcription in Single Living Cells11:22

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Published on: August 25, 2011

15.6K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.3K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K