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在COVID-19患者中评估感染相关的长非编码RNAs的表达:一项病例对照研究
Zahra Firoozi1,2, Elham Mohammadisoleimani2,3,4, Farzaneh Bagheri2,5
1Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.
Genetics research
|February 23, 2024
概括
这项研究研究了COVID-19患者的长非编码RNA (lncRNAs). 在患者中,三个lncRNAs (ISR,ATV,HEAL) 显著升高,IncATV显示为SARS-CoV-2感染的生物标志物.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 引发了涉及细胞因子和化学因子的免疫反应.
- 长非编码RNAs (lncRNAs) 与炎症途径有关,但它们在SARS-CoV-2感染中的特定作用需要进一步阐明.
- 之前的研究集中在病毒感染中的有限数量的lncRNAs上,在了解它们在COVID-19中的功能方面存在差距.
研究的目的:
- 研究 SARS-CoV-2 感染个体外围血液单核细胞 (PBMC) 中特定 lncRNAs 的表达模式.
- 探索这些lncRNAs作为生物标志物的潜力,以区分COVID-19患者与健康个体.
- 在SARS-CoV-2的背景下,识别潜在的竞争性内源RNA (ceRNA) 网络,涉及lncRNAs.
主要方法:
- 从53名COVID-19患者和45名健康对照组中收集了血液样本.
- 分离了外围血液单核细胞 (PBMCs) 用于RNA提取和补充DNA (cDNA) 合成.
- 实时PCR被用于评估五种lncRNAs (lnc ISR, lnc ATV, lnc PAAN, lnc SG20和 lnc HEAL) 的表达水平.
- 进行了接收器操作特征 (ROC) 曲线分析,以评估生物标志物潜力.
主要成果:
- 三种lncRNAs,即Inc ISR,Inc ATV和Inc HEAL,在COVID-19患者中表达明显更高,与健康对照人群相比.
- ROC曲线分析表明,Inc ATV在曲线下具有较高的面积 (AUC),这表明它作为区分COVID-19病例的生物标志物的实用性.
- 生物信息分析确定了涉及这些lncRNAs的ceRNA网络,突出了mRNA和miRNA,这些mRNA和miRNA对COVID-19病变产生至关重要.
结论:
- 提高ISR,ATV和HEAL lncRNAs的表达,以及显著的AUC值,表明它们在调节宿主对SARS-CoV-2的天生的免疫反应方面的潜在作用.
- 这些lncRNAs可能有助于调节SARS-CoV-2感染中的病毒复制.
- 需要进一步的体外和体内研究来证实这些lncRNAs在COVID-19中的确切作用和机制.
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