相关实验视频
Updated: Jun 19, 2026
13:06
Viral Tracing of Genetically Defined Neural Circuitry
Published on: October 17, 2012
18.5K
关键非淋巴细胞组织中的PRRSV-2病毒载量与妊娠晚期胎儿妥协有关
1Department of Animal Sciences, Purdue University, West Lafayette, IN, United States.
Frontiers in microbiology
|February 23, 2024
概括
晚期怀孕 猪生殖和呼吸系统综合征病毒2 (PRRSV-2) 感染对胎儿的影响有所不同. PRRSV-2 在胎儿非淋巴细胞组织中分布广泛,敏感胎儿的病毒载量较高,与较差的结果相关.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 胎儿的发育 胎儿的发育
背景情况:
- 在妊娠晚期感染猪生殖和呼吸系统综合征病毒2 (PRRSV-2) 导致胎儿结果变化.
- 了解组织特异性病毒分布和宿主反应对于解释这种变异性至关重要.
研究的目的:
- 研究PRRSV-2在非淋巴瘤胎儿组织中的易感性,分布和影响.
- 为了将特定组织中的病毒载量和免疫反应与胎儿结果 (耐药,弹性,易感) 相关联.
主要方法:
- 在妊娠86.6日时对胎儿的大脑,心脏,脏,肝脏,肺和骨肌的分析.
- 免疫组织光检测用于识别CD163+细胞.
- 在感染胎儿的组织中进行定量PCR (qPCR),体外病毒恢复和基因表达分析 (IFNG,CD163).
主要成果:
- 休息的CD163+细胞存在于所有检查的非淋巴瘤胎儿组织中.
- 在所有六个高度感染胎儿的组织中检测到PRRSV-2RNA和传染性病毒.
- 敏感的胎儿在心脏,大脑,肺部和骨肌肉中显示出明显更高的病毒RNA,与可行的同行相比.
- 感染增加了大脑,和肺部的CD163表达,以及整个组织的IFNG表达,但与敏感性无关.
结论:
- 在敏感的胎儿非淋巴瘤组织中,PRRSV-2广泛传播.
- 在关键胎儿器官中的局部病毒载荷与妊娠晚期感染后的胎儿结果有关.
相关概念视频
01:27Cytomegalovirus Disease
Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
01:29Respiratory Syncytial Virus Disease
Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...