在基于WGCNA的结核病中确定重要的模块和生物标志物
Jing Dong1,2,3, Ruixue Song1,2,3, Xuetian Shang1,2,3
1Beijing Chest Hospital, Capital Medical University, Beijing, China.
Frontiers in microbiology
|February 23, 2024
概括
这项研究确定了新型长非编码RNA (lncRNAs) 作为结核病 (TB) 诊断的潜在生物标志物. 这些发现突出了特定的lncRNAs,可以有效地区分结核病患者和健康人群,有助于早期检测.
科学领域:
- 基因组学和分子生物学
- 传染性疾病 传染性疾病
- 生物标志物发现发现
背景情况:
- 结核病 (TB) 是一个重大的全球卫生挑战,诊断生物标志物有限.
- 长非编码RNAs (lncRNAs) 为结核病的病理洞察力和诊断应用提供了潜力.
- 目前关于结核病中lncRNA表达特征的数据很少.
研究的目的:
- 为了研究结核病患者的lncRNA表达特征.
- 确定关键的lncRNA和与结核病原发生相关的共表达模块.
- 为了验证潜在的lncRNA生物标志物用于结核病诊断.
主要方法:
- 在外围血液单核细胞 (PBMCs) 中分析ceRNA-microarray分析以描述lncRNAs.
- 权重基因共同表达网络分析 (WGCNA) 以确定结核病相关模块.
- 生物信息学分析 (GO,KEGG) 用于功能丰富.
- 实时定量聚合酶连锁反应 (qPCR) 用于生物标志物验证.
- 用于诊断性能评估的接收器操作特征 (ROC) 分析.
主要成果:
- 在结核病患者和健康对照人群中,确定了1372个差异表达的lncRNAs (738个上调,634个下调).
- WGCNA揭示了一种与结核病相关的显著"蓝色模块",丰富了免疫和感染途径.
- 八个候选 lncRNAs 得到了验证,其中ABHD17B 和 ENST00000607464.1 显示出完美的诊断准确度 (AUC=1,000).
结论:
- 结核病患者的 lncRNA 表达特征.
- 确定了与结核病相关的关键 lncRNA 同表达模块.
- 发现了新的lncRNA生物标志物,ABHD17B和ENST00000607464.1,具有结核病诊断的高潜力.
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