瓜子Fom-1-Prv抗性基因对:与病毒蛋白的相关空间表达和相互作用
Michael Normantovich1, Arie Amitzur1, Sharon Offri1
1The Mina and Everard Goodman Faculty of Life Sciences Bar Ilan University Ramat Gan Israel.
Plant direct
|February 23, 2024
概括
瓜子的抗性基因Fom-1和Prv,编码TIR-NBS-LRR蛋白质,提供对Fusarium枯和木瓜环斑病毒的防御. 它们的协调表达和Prvv.
科学领域:
- 植物病理学 植物病理学
- 分子遗传学 分子遗传学
- 植物病原体相互作用
背景情况:
- 瓜子耐药性 (R) 基因Fom-1和Prv赋予了对Fusarium oxysporum和木瓜环状斑点病毒 (PRSV) 的耐药性.
- 这些基因编码Toll-Interleukin-like核酸结合性氨酸丰富重复 (TIR-NBS-LRR) 蛋白质,其中Prv拥有额外的域.
- 配对的R蛋白通常通过"分工"机制起作用,涉及直接的病原体效应因子结合和随后的防御激活.
研究的目的:
- 研究瓜子中的Fom-1和Prv抗性基因的表达模式和功能相互作用.
- 阐明基因间区域在Fom-1和Prv.协调表达中的作用.
- 为了确认这些配对的R蛋白的"分工"模型.
主要方法:
- 在接近同位素的瓜子系中对Fom-1和Prv基因表达的定量分析.
- 在转基因西瓜根和烟草植物中进行GUS记者基因测试,以研究促进体活性.
- 酵母两杂交试验和植物共免疫沉以研究蛋白质相互作用.
主要成果:
- Fom-1和Prv表达水平在抗性西瓜系中较高,并且可由Fusarium oxysporum种族2诱导.
- 基因间DNA区域充当了双向促进器的功能,驱动了特定根和血管组织中的GUS表达.
- Prv的额外域直接与酵母和植物中的PRSV圆柱状包容蛋白相互作用.
结论:
- Fom-1和Prv基因表现出协调表达,由共享的双向促进体调节.
- 结果支持"分工"模型,其中Prv的额外域与PRSV效应器直接相互作用.
- 这项研究提供了植物对真菌和病毒病原体耐药性的分子机制的见解.
关键词:
这就是子子 (Cucumis melo).这份表格是Form‐1.体氧化体 (Fusarium oxysporum) 是一种体.普利斯维加斯 普利斯维加斯这就是为什么Prvv这对R基因对.圆柱状的包容蛋白质是一种圆柱状的包容蛋白质.集成的诱.瓜子 瓜子 瓜子更多相关视频
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