相关实验视频

Updated: Jul 2, 2025

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

先进的制造提供了定制的解决方案,用于用X射线自由电子激光器进行晶体学

Lars Paulson¹, Sankar Raju Narayanasamy², Megan L Shelby²

¹Department of Chemistry & Research and Education in Energy, Environment and Water (RENEW), The State University of New York at Buffalo, Buffalo, New York 14260, USA.

Structural dynamics (Melville, N.Y.)

|February 23, 2024

概括

3D打印技术的进步提高了连续晶体学的微流体学,使蛋白质微晶能够有效地处理. 这有助于进行高分辨率的结构研究,这对药物发现和开发新疗法至关重要.

科学领域:

结构生物学结构生物学
生物物理学的生物物理.
材料科学材料科学材料科学

背景情况:

序列晶体学对于高分辨率的蛋白质结构确定至关重要.
高效的微观样本处理是串行晶体学的一个主要挑战.
微流体学为高通量,灵活的样本操纵提供了一个解决方案.

研究的目的:

审查最近在3D打印中取得的进展,用于串行结晶学中的微流体.
为了展示新兴的纳米3D打印技术用于样品传递设备.

主要方法:

对微流体设备的聚合物3D打印进行讨论.
展示先进的纳米3D打印用于样本环境制造.
探索液体喷气气体动态虚拟喷嘴和固定目标样本的交付.

主要成果:

3D打印为微流体设备提供了设计灵活性和快速原型设计.
新兴的纳米3D打印使复杂的样品环境和交付系统成为可能.
新的制造方法解决了处理蛋白质微晶的挑战.

结论:

3D打印正在为串行晶体学进行微流体学革命.
先进的3D打印技术正在为未来的样品交付创新铺平道路.
这些进展将加速蛋白质结构的确定和药物发现.

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相关概念视频

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...