由含有流感双血凝素和M2外原蛋白的病毒样颗粒诱导的交叉保护
Jie Mao1, Gi-Deok Eom1, Keon-Woong Yoon1
1Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea.
Nanomedicine (London, England)
|February 23, 2024
概括
使用表达双重血凝素 (H1/H3) 和M2e5x的病毒样颗粒 (VLP) 开发通用流感疫苗显得有前途. 这一策略增强了对各种流感A病毒菌株的交叉保护性免疫力.
科学领域:
- * 病毒学 病毒学
- * 免疫学 免疫学
- * 疫苗学 疫苗学 疫苗学
背景情况:
- * 由于不断的抗原漂移和转移,A型流感病毒对全球健康构成重大威胁.
- *目前的疫苗往往需要经常更新,以匹配流通的菌株.
- *开发一种通用疫苗仍然是传染病研究的关键目标.
研究的目的:
- * 为了设计表达H1和H3血凝素抗原的流感病毒样颗粒 (VLPs).
- * 评估具有M2e5x表位组和没有M2e5x表位组的VLPs的免疫性和保护效果.
- * 评估这些VLP作为对抗原性多样性流感病毒的通用疫苗策略的潜力.
主要方法:
- * 呈现H1和H3血凝素的流感VLP的生成,其中包括M2e5x表位的变异.
- *评估VLP诱导的免疫反应,包括IgG抗体标位和生殖中心B细胞活性.
- * 在体内对与H1N1,H3N2和H5N1流感病毒的挑战进行交叉保护的评估.
主要成果:
- *与H1H3M2e5xVLPs免疫接种诱导了与H1H3VLPs相比更高的交叉反应性IgG抗体.
- * 两种VLP配方都提高了挑战后的肺部IgG,IgA和生殖中心B细胞反应.
- *与H1H3M2e5x VLPs相比,H1H3M2e5x VLPs显示肺病毒载荷的降低优异,并最大限度地减少了体重减轻.
结论:
- * 呈现双重血凝素 (H1/H3) 和M2e5x表位的病毒样颗粒代表了广泛流感A病毒保护的可行策略.
- *这种基于VLP的方法可以诱导强大的免疫反应,并提供显著的交叉保护.
- *这些发现支持开发含有M2e5x的双血凝素VLPs作为潜在的通用流感疫苗候选人.
更多相关视频
04:47A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Published on: February 23, 2018
7.7K
08:10Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
Published on: January 15, 2020
8.3K
相关概念视频
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
00:42Cross-reactivity
31.1K
Overview
31.1K
01:29Immune Response Against Viral Pathogens
782
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
782
00:57Viral Recombination
23.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.4K