相关实验视频

Updated: Jul 2, 2025

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

单细胞和批量RNA测序数据的综合分析揭示了与Mycobacterium tuberculosis延迟相关的类似记忆的NK细胞子集

Mojtaba Shekarkar Azgomi^1,2, Giusto Davide Badami¹, Marianna Lo Pizzo¹

¹Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), Azienda Ospedaliera Universitaria Policlinico (AOUP) Paolo Giaccone, University of Palermo, 90127 Palermo, Italy.

|February 23, 2024

概括

自然杀手 (NK) 细胞在结核病免疫力中起作用. 潜在的Mycobacterium结核病感染显示了特定NK细胞种群的增加,这表明在保护性反应中发挥了作用.

科学领域:

免疫学免疫学免疫学
传染性疾病传染性疾病
细胞生物学细胞生物学

背景情况:

自然杀手 (NK) 细胞对于对病毒和瘤细胞的天生的免疫力至关重要.
对于NK细胞对抗细胞内微生物 (如Mycobacterium tuberculosis (Mtb)) 的保护作用尚不清楚.

研究的目的:

定义外围血液NK细胞的分子和表型特征.
研究活跃结核病 (TB),潜伏的Mtb感染 (LTBI) 和其他肺部疾病中的NK细胞反应.

主要方法:

结合scRNA-seq和大量RNA-seq数据分析.
在患者和健康捐赠者的队列中进行流细胞计验证.

主要成果:

与活跃结核病,其他疾病和健康捐赠者相比,LTBI患者的NK细胞显著增加.
这种增加主要是由于表达KLRC2,CD52,CCL5和HLA-DRB1的NK细胞的扩张,类似于记忆类NK2.1细胞.
在LTBI受试者中,记忆类CD52+NKG2C+NK细胞显著扩张.

结论:

在潜伏的Mtb感染中,NK细胞,特别是类似记忆的群体,会扩大.
这些发现为NK细胞在对MTB的保护性免疫力中的功能提供了新的见解.

关键词:

结核病菌菌菌的结核病菌的结核病菌.在 NK 细胞中,NK 细胞是 NK 细胞.在 NKG2C 中,NKG2C 是 NKG2C 的代码.潜伏的结核菌菌菌菌的感染.单细胞RNA序列的一个单细胞RNA序列.结核病是一种肺结核病.

更多相关视频

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

相关概念视频

Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...