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相关概念视频

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
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用原子力显微镜获取中性粒细胞激活水平的细胞表面参数.

Oksana M Tilinova1, Vladimir Inozemtsev2, Ekaterina Sherstyukova2

  • 1Sarov Physics and Technology Institute, MEPhI, 607186 Sarov, Russia.

Cells
|February 23, 2024
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概括

中性粒细胞激活涉及在30分钟内显著的细胞表面变化,可能是由于反应性氧物种. 原子力显微镜揭示了早期的颗粒结构,并允许使用表面参数估计激活度.

关键词:
响声模式 响声模式激活方式 激活方式粘附性 粘附性 粘附性 粘附性原子力显微镜的原子力显微镜.细胞表面的细胞表面.葡萄糖糖的性糖质 (glycocalyx) 是一种作为一个中性恋者,有活性氧的反应氧.

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科学领域:

  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学
  • 生物物理学的生物物理.

背景情况:

  • 中性粒细胞是关键的免疫细胞,参与炎症反应.
  • 了解中性粒细胞激活动态是研究免疫功能和疾病的关键.
  • 细胞表面的地形和粘附特性在中性粒细胞激活过程中发生变化.

研究的目的:

  • 为了研究在激活过程中中性粒细胞表面的地形和粘附变化.
  • 为了确定与中性粒细胞激活时间相关的关键表面参数.
  • 用原子力显微镜建立估计中性粒细胞激活的方法.

主要方法:

  • 利用原子力显微镜 (AFM) 绘制中性粒细胞表面的地形和粘附.
  • 使用共聚焦激光扫描显微镜观察内部细胞重组.
  • 分析了细胞表面参数及其与激活时间的相关性.

主要成果:

  • 在激活后的前30分钟内观察到显著的中性粒细胞表面变化.
  • 通过AFM激活后10分钟,表面颗粒结构就可见了.
  • 确定了三个与中性粒细胞激活时间相关的空间表面参数.

结论:

  • 中性粒细胞激活是一种具有快速细胞表面变化的动态过程.
  • AFM成像为中性粒细胞激活机制提供了宝贵的见解.
  • 鉴定到的表面参数允许对中性粒细胞激活度进行定量估计.