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Updated: Jul 2, 2025

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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参考基因的季节性稳定性评估用于Bombus terrestris中的定量实时聚合酶连锁反应规范化

Kathannan Sankar1,2, Kyeong-Yong Lee1, Kyu-Won Kwak1

  • 1Agricultural Biology Department, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.

Current issues in molecular biology
|February 23, 2024
PubMed
概括

这项研究确定了最佳的参考基因,用于将熊蜂 (Bombus terrestris) 的基因表达数据正常化. 这些发现对于理解季节性适应和改进大黄蜂管理策略至关重要.

关键词:
在RefFinder中找到.基因表达的基因表达方式基因验证 基因验证年轻人荷尔蒙的激素.qRT-PCR 是一个很好的方法.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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